Abstract |
Mice vaccinated by injection with tumor cells expressing the Mycobacterium leprae gene for hsp65 acquire a remarkably high degree of protection against challenge with Mycobacterium tuberculosis. We used limiting-dilution analysis to assess the frequency of CD4+ CD8- and CD4- CD8+ splenocytes responding to mycobacterial hsp65 in such vaccinated mice. Cells of both phenotypes were present at very high and equal frequencies (approximately 1:100). Vaccination with live Mycobacterium bovis BCG also increased the frequencies of both phenotypes of hsp65-reactive cells equally (to approximately 1:2,500), whereas vaccination procedures that were not protective, with either dead BCG, hsp65 protein in incomplete Freund's adjuvant, or hsp65 mixed with tumor cells, resulted in preferential increase in CD4+ CD8- cells. Twelve CD4+ CD8- and twelve CD4- CD8+ hsp65-responsive T-cell clones were obtained and characterized. All showed conventional antigen recognition via major histocompatibility complex class II and class I pathways but differed in secretion of gamma interferon and interleukin 4 and cytotoxicity. In tests of antimycobacterial activity against M. tuberculosis, both in infected macrophages in vitro and by adoptive transfer of protection with T-cell clones injected into irradiated mice, the most effective clones were the most cytotoxic and secretion of gamma interferon made only a secondary contribution.
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Authors | C L Silva, M F Silva, R C Pietro, D B Lowrie |
Journal | Infection and immunity
(Infect Immun)
Vol. 64
Issue 7
Pg. 2400-7
(Jul 1996)
ISSN: 0019-9567 [Print] United States |
PMID | 8698458
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antigens, Bacterial
- BCG Vaccine
- Bacterial Proteins
- Chaperonin 60
- Cytokines
- heat-shock protein 65, Mycobacterium
- Chaperonins
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Topics |
- Animals
- Antigens, Bacterial
- BCG Vaccine
(immunology)
- Bacterial Proteins
(immunology)
- CD4-Positive T-Lymphocytes
(immunology)
- CD8-Positive T-Lymphocytes
(immunology)
- Chaperonin 60
- Chaperonins
(immunology)
- Cytokines
(biosynthesis)
- Cytotoxicity, Immunologic
- Immunotherapy, Adoptive
- Mice
- Mice, Inbred BALB C
- Mice, Inbred C57BL
- Mycobacterium leprae
(immunology)
- Neoplasms, Experimental
(immunology)
- T-Lymphocytes
(immunology)
- Tuberculosis
(immunology, prevention & control)
- Vaccination
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