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Effect of oncotic pressure of diaspirin cross-linked hemoglobin (DCLHb) on brain injury after temporary focal cerebral ischemia in rats.

Abstract
Previous studies have shown that diaspirin cross-linked hemoglobin (DCLHb, 10 g/dL) decreases cerebral ischemia and the resultant injury in a dose-dependent manner, requiring large volumes of DCLHb for maximum efficacy. We assessed the effect of a more concentrated (20 g/dL) and more hyperoncotic preparation of DCLHb on cerebral infarction volume. Immediately after middle cerebral artery occlusion, rats were randomized to one of the following groups: Control, hematocrit not manipulated; 10/Hb, hematocrit decreased to 30% with 10% DCLHb (oncotic pressure 43 mm Hg); 7.5/Alb, hematocrit decreased to 30% with 7.5% albumin (oncotic pressure 43 mm Hg); 20/Hb, the same dose of DCLHb (20%, oncotic pressure 129 mm Hg) as the 10/HB group (half the volume); or 15/Alb, the same dose of albumin (15%, oncotic pressure 130 mm Hg) as the 7.5/Alb group half the volume). After 90 min of ischemia, 72 h of reperfusion was allowed. Infarction volume (mm3, mean +/- sd) was less in the DCLHb groups (10/Hb = 79 +/- 17; 20/HB = 51 +/- 14) than the oncotically matched albumin groups (7.5/Alb = 124 +/- 21; 15/Alb = 85 +/- 18) and the Control group (135 +/- 17) (P < 0.05). These data indicate that in this model of cerebral ischemia, DCLHb decreases ischemic brain injury more effectively than albumin, and that a hyperoncotic preparation of DCLHb is preferable.
AuthorsD J Cole, J C Drummond, P M Patel, J C Nary, R L Applegate 2nd
JournalAnesthesia and analgesia (Anesth Analg) Vol. 83 Issue 2 Pg. 342-7 (Aug 1996) ISSN: 0003-2999 [Print] United States
PMID8694316 (Publication Type: Journal Article)
Chemical References
  • Coloring Agents
  • Hemoglobins
  • Serum Albumin
  • Tetrazolium Salts
  • triphenyltetrazolium
  • diaspirin-cross-linked hemoglobin
  • Aspirin
Topics
  • Animals
  • Aspirin (administration & dosage, analogs & derivatives, therapeutic use)
  • Blood Volume
  • Brain (drug effects, pathology)
  • Cerebral Infarction (pathology, prevention & control)
  • Coloring Agents
  • Dose-Response Relationship, Drug
  • Exchange Transfusion, Whole Blood
  • Hematocrit
  • Hemodilution
  • Hemoglobins (administration & dosage, therapeutic use)
  • Ischemic Attack, Transient (drug therapy)
  • Male
  • Osmotic Pressure
  • Random Allocation
  • Rats
  • Rats, Inbred SHR
  • Reperfusion
  • Serum Albumin (administration & dosage, therapeutic use)
  • Tetrazolium Salts

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