Abstract |
Toluene diisocyanate (TDI)-induced asthma is a common cause of occupational lung disease. We used a model to investigate the course of bronchopulmonary inflammation following immunization with TDI. Guinea pigs were immunized by weekly intradermal injections and challenged with TDI 7 d after the third injection. The animals were killed at different times after challenge and prepared for histologic examination of central and peripheral airways, for immunohistochemical studies of T lymphocyte and eosinophil distribution, and for hematologic and serologic investigations. Specific IgG1 against TDI were present only in immunized animals. In immunized TDI-challenged animals there was a significant increase in the number of metachromatic cells (at 24 h) and a late increase of eosinophils (at 48 h) in the peripheral blood. Mast cells and eosinophils were also increased in the submucosa of central airways of immunized TDI-challenged animals. A similar pattern was observed in the animals' peripheral airways. Additionally, a significant increase of T-lymphocytes and eosinophils was found in the lamina propria at 6 h after exposure in immunized TDI-challenged animals as compared with control animals. In these immunized animals, TDI challenge caused a significant increase of eosinophils, T-lymphocytes, and CD4+ T cells. These findings indicate that intradermal injections of TDI induced a specific antibody response as well as an inflammatory process in both central and peripheral airways. T cells, particularly CD4+ T cells and eosinophils, are the key cells in the immunopathologic alterations induced by TDI in the guinea pig lung.
|
Authors | C E Mapp, J R Lapa e Silva, R E Lucchini, P Chitano, V Rado, M Saetta, M Pretolani, M H Karol, P Maestrelli, L M Fabbri |
Journal | American journal of respiratory and critical care medicine
(Am J Respir Crit Care Med)
Vol. 154
Issue 1
Pg. 201-8
(Jul 1996)
ISSN: 1073-449X [Print] United States |
PMID | 8680681
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Antibodies
- Immunoglobulin G
- Toluene 2,4-Diisocyanate
|
Topics |
- Animals
- Antibodies
(analysis)
- Asthma
(blood, etiology, pathology)
- Blood Cell Count
- Bronchi
(pathology)
- Eosinophils
(pathology)
- Guinea Pigs
- Immunization
- Immunoglobulin G
(analysis)
- Immunohistochemistry
- Inflammation
(pathology)
- Lung
(pathology)
- Male
- Mast Cells
(pathology)
- Toluene 2,4-Diisocyanate
(immunology)
|