Selective control of cytosolic glutathione peroxidase and phospholipid hydroperoxide glutathione peroxidase mRNA stability by selenium supply.

Abstract

Selenium depletion of H4 hepatoma cells reduced cytosolic glutathione peroxidase (cGSH-Px) mRNA abundance but had no effect on phospholipid hydroperoxide glutathione peroxidase (PHGSH-Px) mRNA abundance. Actinomycin D chase experiments showed that selenium depletion had no effect on the stability of PHGSH-Px mRNA but decreased the stability of cGSH-Px mRNA. In Se-replete cells puromycin decreased the stability of both cGSH-Px and PHGSH-Px mRNAs. The results suggest that when selenium supply is limiting PHGSH-Px mRNA translation is maintained more than that of cGSH-Px mRNA, and thus more cGSH-Px mRNA is released from polysomes and degraded.

• Authors: G Bermano, J R Arthur, J E Hesketh
• Journal: FEBS letters (FEBS Lett) Vol. 387 Issue 2-3 Pg. 157-60 (Jun 03 1996) ISSN: 0014-5793 [Print] England
• PMID: 8674540 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• RNA, Messenger
• Phospholipid Hydroperoxide Glutathione Peroxidase
• Glutathione Peroxidase
• Selenium

Topics

• Animals
• Cytosol (enzymology)
• Glutathione Peroxidase (genetics, metabolism)
• Phospholipid Hydroperoxide Glutathione Peroxidase
• RNA, Messenger (metabolism)
• Rats
• Selenium (deficiency, metabolism)
• Tumor Cells, Cultured