Abstract |
Human herpesvirus 6 (HHV-6) was examined in vitro for its sensitivity to a broad range of nucleoside analogues, including acyclovir (ACV), ganciclovir (GCV), penciclovir (PCV), buciclovir (BCV), brivudin (BVDU), the N7-isomer of 6-deoxyganciclovir ( S2242), foscarnet ( phosphonoformic acid, PFA), and several acyclic nucleoside phosphonate ( ANP) analogues such as ( S)-HPMPA, (S)- HPMPC, PMEA and PMEDAP. Antiviral efficacy was monitored microscopically by the inhibitory effect of the compounds on HHV-6-induced cytopathic effect in human T-lymphoblastoid HSB-2 cells. In addition, a newly developed immunofluorescence/flow cytometric assay (FACS) was used to determine HHV-6-specific antigen expression. A close correlation was observed between the antiviral data obtained by the microscopic assay and the flow cytometric assay. Marked antiviral efficacy was noted for S2242, PFA and the ANP analogues ( S)-HPMPA, (S)- HPMPC, (S)- cHPMPC, (S)-3-deaza-HPMPA, (S)-3-deaza-cHPMPA, (S)- HPMPG and (R)- HPMPG. Also, PMEA and PMEDAP proved highly active against HHV-6 infection, whereas (S)-FPMPA and (R)- PMPDAP were inactive. ACV was only slightly protective against HHV-6, and no activity was found for GCV, PCV, BCV and BVDU. Overall, the efficacy of the nucleoside analogues against HHV-6 appeared to correlate with their efficacy against human cytomegalovirus (HCMV).
|
Authors | D Reymen, L Naesens, J Balzarini, A Holý, H Dvoráková, E De Clercq |
Journal | Antiviral research
(Antiviral Res)
Vol. 28
Issue 4
Pg. 343-57
(Dec 1995)
ISSN: 0166-3542 [Print] Netherlands |
PMID | 8669893
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Antiviral Agents
- Nucleosides
- Organophosphonates
- Organophosphorus Compounds
- Foscarnet
- 9-(2-phosphonylmethoxyethyl)-2,6-diaminopurine
- Cytosine
- Adenine
- Cidofovir
- Ganciclovir
|
Topics |
- Adenine
(analogs & derivatives, pharmacology)
- Antiviral Agents
(pharmacology)
- Cell Line
- Cidofovir
- Cytopathogenic Effect, Viral
- Cytosine
(analogs & derivatives, pharmacology)
- Flow Cytometry
- Foscarnet
(pharmacology)
- Ganciclovir
(analogs & derivatives, pharmacology)
- Herpesvirus 6, Human
(drug effects)
- Humans
- Nucleosides
(pharmacology)
- Organophosphonates
- Organophosphorus Compounds
(pharmacology)
|