Abstract |
A rat model was used to study the effects of granulocyte colony-stimulating factor ( G-CSF) on the pathogenesis of pneumococcal pneumonia in cirrhosis. G-CSF or 5% dextrose in water was administered subcutaneously to cirrhotic and control rats before or after transtracheal infection with type 3 Streptococcus pneumoniae. In both groups, G-CSF significantly increased the total number and percentage of polymorphonuclear leukocytes (PMNL) in peripheral blood (P < .002) and bronchoalveolar lavage fluid (P < .01). An in vivo phagocytosis assay revealed no increase in uptake of pneumococci by PMNL within the lungs of cirrhotic or control rats receiving G-CSF. G-CSF administered before infection did not protect cirrhotic or control rats, but G-CSF treatment after infection significantly reduced mortality in control (P = .04) but not cirrhotic rats. These data suggest that despite increasing numbers of circulating and pulmonary PMNL, G-CSF does not protect against fatal pneumococcal pneumonia in cirrhotic rats.
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Authors | L C Preheim, M U Snitily, M J Gentry |
Journal | The Journal of infectious diseases
(J Infect Dis)
Vol. 174
Issue 1
Pg. 225-8
(Jul 1996)
ISSN: 0022-1899 [Print] United States |
PMID | 8656001
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
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Chemical References |
- Granulocyte Colony-Stimulating Factor
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Topics |
- Animals
- Disease Models, Animal
- Granulocyte Colony-Stimulating Factor
(administration & dosage, therapeutic use)
- Liver Cirrhosis, Experimental
(complications, physiopathology)
- Neutrophils
(drug effects)
- Phagocytosis
(drug effects)
- Pneumonia, Pneumococcal
(complications, physiopathology, prevention & control)
- Rats
- Time Factors
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