Enhanced glomerular profilin gene and protein expression in experimental mesangial proliferative glomerulonephritis.

Abstract	Profilin is a cytoplasmic protein that binds to actin monomer and regulates actin polymerization. In a number of experimental and human glomerular diseases, the mesangial cell expresses alpha-smooth muscle actin and undergoes a phenotypic change to myofibroblast. We used a rat model of mesangial proliferative nephritis induced with antibody to the Thy 1 antigen present on mesangial cells to investigate whether profilin is upregulated. We amplified and sequenced rat profilin cDNA by the reverse-transcribed-polymerase chain reaction (RT-PCR). The nucleotide and amino acid sequences were highly conserved across the mammalian profilins. We raised affinity purified antibody to rat profilin in rabbits immunized with a synthetic profilin peptide (EFTMDLRTKS). At 7 days after disease induction, enhanced expression in both profilin mRNA and protein was demonstrated in the isolated glomeruli by RT-PCR and Western blot analysis. These results suggest that profilin may be involved in the pathogenesis of glomerulonephritis by reorganizing actin cytoskeleton.
Authors	M Tamura, H Tanaka, T Hirano, Y Ueta, K Higashi, H Hirano
Journal	Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 222 Issue 3 Pg. 683-7 (May 24 1996) ISSN: 0006-291X [Print] United States
PMID	8651905 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Contractile Proteins Microfilament Proteins PFN1 protein, human Peptides Pfn1 protein, mouse Pfn1 protein, rat Profilins RNA, Messenger
Topics	Amino Acid Sequence Animals Base Sequence Cloning, Molecular Contractile Proteins Gene Expression Glomerular Mesangium (metabolism, pathology) Glomerulonephritis, Membranoproliferative (metabolism, pathology) Humans Mice Microfilament Proteins (metabolism) Molecular Sequence Data Peptides (immunology) Profilins RNA, Messenger (genetics) Rats Rats, Wistar Sequence Alignment Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid

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