Therapeutic effectiveness of the immunity elicited by P815 tumor cells engineered to express the B7-2 costimulatory molecule.

Abstract

It is well accepted that inoculation of B7-1-transfected tumor cells into normal mice leads to tumor rejection and subsequent resistance to challenge. However, the effectiveness of B7-2-transfected tumor cells in eliciting protective antitumor immunity is less clear. Here we show that B7-2-transfected P815 tumor cells (B7-2+) are as effective as B7-1-transfected P815 tumor cells (B7-1+) in eliciting protective immunity in normal DBA/2 mice. In addition, B7-2+ cells were found to be at least as effective as B7-1+ cells retarding tumor progression when admixed with parental P815 tumor cells prior to inoculation into normal mice. Moreover, the B7-2+ cells and the B7-1+ cells were equivalent in their ability to retard tumor growth when administered peritumorally into mice bearing established (approx. 3 mm in diameter) parental P815 tumors. Finally, P815 tumor cells infected with a recombinant replication-defective adenovirus encoding the murine B7-2 gene were effective in retarding the growth of established parental P815 tumors. Thus, B7-1 and B7-2 are comparable in terms of their ability to stimulate the generation of tumor-eradicating immunity in normal mice as well as in mice bearing established parental tumors. Moreover, adenovirus vectors can be used to generate B7-2-expressing tumor cells effective in the immunotherapy of established parental tumors.

• Authors: R N La Motte, M A Rubin, E Barr, J M Leiden, J A Bluestone, M B Mokyr
• Journal: Cancer immunology, immunotherapy : CII (Cancer Immunol Immunother) Vol. 42 Issue 3 Pg. 161-9 (Mar 1996) ISSN: 0340-7004 [Print] Germany
• PMID: 8640844 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antigens, CD
• B7-2 Antigen
• Cd86 protein, mouse
• Membrane Glycoproteins

Topics

• Adenoviridae (genetics)
• Animals
• Antigens, CD (biosynthesis, genetics)
• B7-2 Antigen
• Female
• Immunotherapy, Active
• Mast-Cell Sarcoma (genetics, immunology, therapy)
• Membrane Glycoproteins (biosynthesis, genetics)
• Mice
• Mice, Inbred DBA
• Transfection