Abstract |
We investigated the in vitro and in vivo effects of recombinant human granulocyte colony-stimulating factor (rhG-CSF) on the proliferation of two murine leukemic cell lines. The rhG-CSF stimulated leukemic colony formation of the promyelocytic leukemic cell line L-8801 in methylcellulose culture and increased the number of L-8801 cells in liquid culture. However, rhG-CSF treatment prolonged the median survival period of mice implanted with L-8801 cells and the emergence of the leukemic blast cells in peripheral blood. Meanwhile, rhG-CSF had no influence on that of the megakaryoblastic leukemic cells L-8057 and failed to prolong the median survival period of L-8057 leukemic mice. Receptor binding analysis revealed that L-8801 cells expressed a G-CSF receptor (Kd=125 pM, 479 binding sites/cell) and L-8057 cells had no G-CSF receptors. Then, we examined the growth potential of these cells. The median survival period was longer for mice implanted with L-8801 cells cultured with rhG-CSF for 72 h in vitro than for cells grown without rhG-CSF. Furthermore, the median survival period of mice implanted with spleen cells from L-8801 leukemic mice treated with rhG-CSF was prolonged compared with those from leukemic mice without rhG-CSF. In contrast, there was no effect of rhG-CSF on the growth potential of the spleen from L-8057 leukemic mice. The results of our present study demonstrate that rhG-CSF reduced the growth of L-8801 leukemic cells in vitro and in vivo mediated through G-CSF receptors, thereby suppressing the development of leukemia.
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Authors | K Kabaya, M Obuchi, T Kuwaki, K Shibuya, M Watanabe, K Nemoto, K Yoshida, M Kusaka, T Inoue, M Seki |
Journal | Leukemia research
(Leuk Res)
Vol. 20
Issue 1
Pg. 27-35
(Jan 1996)
ISSN: 0145-2126 [Print] England |
PMID | 8632675
(Publication Type: Journal Article)
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Chemical References |
- Receptors, Granulocyte Colony-Stimulating Factor
- Recombinant Proteins
- Granulocyte Colony-Stimulating Factor
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Topics |
- Animals
- Cell Survival
(drug effects)
- Granulocyte Colony-Stimulating Factor
(metabolism, pharmacology)
- Humans
- Leukemia, Myeloid
(pathology, therapy)
- Male
- Mice
- Mice, Inbred C3H
- Receptors, Granulocyte Colony-Stimulating Factor
(analysis)
- Recombinant Proteins
(pharmacology)
- Tumor Cells, Cultured
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