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Effects of recombinant human granulocyte colony-stimulating factor on the growth potential of two murine myeloid leukemias.

Abstract
We investigated the in vitro and in vivo effects of recombinant human granulocyte colony-stimulating factor (rhG-CSF) on the proliferation of two murine leukemic cell lines. The rhG-CSF stimulated leukemic colony formation of the promyelocytic leukemic cell line L-8801 in methylcellulose culture and increased the number of L-8801 cells in liquid culture. However, rhG-CSF treatment prolonged the median survival period of mice implanted with L-8801 cells and the emergence of the leukemic blast cells in peripheral blood. Meanwhile, rhG-CSF had no influence on that of the megakaryoblastic leukemic cells L-8057 and failed to prolong the median survival period of L-8057 leukemic mice. Receptor binding analysis revealed that L-8801 cells expressed a G-CSF receptor (Kd=125 pM, 479 binding sites/cell) and L-8057 cells had no G-CSF receptors. Then, we examined the growth potential of these cells. The median survival period was longer for mice implanted with L-8801 cells cultured with rhG-CSF for 72 h in vitro than for cells grown without rhG-CSF. Furthermore, the median survival period of mice implanted with spleen cells from L-8801 leukemic mice treated with rhG-CSF was prolonged compared with those from leukemic mice without rhG-CSF. In contrast, there was no effect of rhG-CSF on the growth potential of the spleen from L-8057 leukemic mice. The results of our present study demonstrate that rhG-CSF reduced the growth of L-8801 leukemic cells in vitro and in vivo mediated through G-CSF receptors, thereby suppressing the development of leukemia.
AuthorsK Kabaya, M Obuchi, T Kuwaki, K Shibuya, M Watanabe, K Nemoto, K Yoshida, M Kusaka, T Inoue, M Seki
JournalLeukemia research (Leuk Res) Vol. 20 Issue 1 Pg. 27-35 (Jan 1996) ISSN: 0145-2126 [Print] England
PMID8632675 (Publication Type: Journal Article)
Chemical References
  • Receptors, Granulocyte Colony-Stimulating Factor
  • Recombinant Proteins
  • Granulocyte Colony-Stimulating Factor
Topics
  • Animals
  • Cell Survival (drug effects)
  • Granulocyte Colony-Stimulating Factor (metabolism, pharmacology)
  • Humans
  • Leukemia, Myeloid (pathology, therapy)
  • Male
  • Mice
  • Mice, Inbred C3H
  • Receptors, Granulocyte Colony-Stimulating Factor (analysis)
  • Recombinant Proteins (pharmacology)
  • Tumor Cells, Cultured

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