Interaction of NMDA and dopamine D2L receptors in human neuroblastoma SH-SY5Y cells.

Abstract

To understand the mechanism of interaction of the dopamine D2L receptors with NMDA receptors, we have developed a model by transfecting human neuroblastoma SH-SY5Y cells with the human dopamine D2L receptor gene. In vitro blockade of NMDA receptors by the specific antagonists MK-801 and (+/-)-3-(2-carboxypiperazin-4-yl)-propyl-1-phosphonic acid (CPP) on human neuroblastoma SH-SY5Y cells expressing human dopamine D2L receptors resulted in a significant increase in the density of D2L receptors without a significant change in receptor affinity. Moreover, the dopamine receptor mRNA level increased by approximately 50% by the blockade of NMDA with MK-801. These results suggest a possible interaction of NMDA and dopamine D2L receptors in neuroblastoma SH-SY5Y cells. This system would serve as an excellent model to study the molecular mechanisms involved in the interaction of these two receptors.

• Authors: V D Nair, H B Niznik, R K Mishra
• Journal: Journal of neurochemistry (J Neurochem) Vol. 66 Issue 6 Pg. 2390-3 (Jun 1996) ISSN: 0022-3042 [Print] England
• PMID: 8632161 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Excitatory Amino Acid Antagonists
• Piperazines
• RNA, Messenger
• Receptors, Dopamine D2
• Receptors, N-Methyl-D-Aspartate
• Dizocilpine Maleate
• 3-(2-carboxypiperazin-4-yl)propyl-1-phosphonic acid

Topics

• Base Sequence
• Dizocilpine Maleate (pharmacology)
• Excitatory Amino Acid Antagonists (pharmacology)
• Humans
• Molecular Sequence Data
• Neuroblastoma
• Piperazines (pharmacology)
• Polymerase Chain Reaction
• RNA, Messenger (analysis)
• Receptors, Dopamine D2 (genetics, physiology)
• Receptors, N-Methyl-D-Aspartate (antagonists & inhibitors, physiology)
• Transfection
• Tumor Cells, Cultured (chemistry)
• Up-Regulation (physiology)