Cloning and tissue distribution of the human P2Y1 receptor.

Abstract

Sets of degenerate oligonucleotide primers synthesized on the basis of the best conserved regions of the chick brain P2Y/P2Y1 and the murine neuroblastoma P2U/P2Y2 receptors were used in polymerase chain reaction experiments on human genomic DNA. An amplified fragment of 712 base pairs was then used as a probe to screen a human genomic DNA library. Several clones were isolated and sequencing revealed an intronless 1122 base pair open reading frame. The corresponding amino acid sequence revealed 83% identity with the chick brain P2Y1 receptor and 34% with the murine neuroblastoma P2Y2 receptor. In COS-7 cells transfected with the coding sequence inserted into the pcDNA3 expression vector, 2-methylthioATP and ATP produced a strong stimulation of inositol phosphates, a typical response of a P2Y1 receptor. Northern blot analysis detected a 6.7 kilobase messenger RNA in most human tissues, the strongest signals being observed in prostate and ovary.

• Authors: R Janssens, D Communi, S Pirotton, M Samson, M Parmentier, J M Boeynaems
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 221 Issue 3 Pg. 588-93 (Apr 25 1996) ISSN: 0006-291X [Print] United States
• PMID: 8630005 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• P2RY1 protein, human
• P2ry1 protein, mouse
• Receptors, Purinergic P2
• Receptors, Purinergic P2Y1
• DNA

Topics

• Amino Acid Sequence
• Animals
• Base Sequence
• Blotting, Northern
• Cell Line
• Chickens
• Cloning, Molecular
• DNA
• Humans
• Mice
• Molecular Sequence Data
• Receptors, Purinergic P2 (genetics, metabolism)
• Receptors, Purinergic P2Y1
• Tissue Distribution
• Tumor Cells, Cultured