Abstract |
The chlL gene product is involved in the light-independent synthesis of chlorophyll in photosynthetic bacteria, green algae and non-flowering plants. The chloroplast genome of Chlorella vulgaris strain C-27 contains the first example of a split chlL gene, which is interrupted by 951 bp group I intron in the coding region. In vitro synthesized pre-mRNA containing the entire intron and parts of the flanking exon sequence is able to efficiently self-splice in vitro in the presence of a divalent and a monovalent cation and GTP, to yield the ligated exons and other splicing intermediates characteristic of self-splicing group I introns. The 5' and 3' splice sites were confirmed by cDNA sequencing and the products of the splicing reaction were characterized by primer extension analysis. The absence of a significant ORF in the long P9 region (522 nt), separating the catalytic core from the 3' splice site, makes this intron different from the other known examples of group I introns. Guanosine-mediated attack at the 3' splice site and the presence of G-exchange reaction sites internal to the intron are some other properties demonstrated for the first time by an intron of a protein-coding plastid gene.
|
Authors | M Kapoor, T Wakasugi, K Yoshinaga, M Sugiura |
Journal | Molecular & general genetics : MGG
(Mol Gen Genet)
Vol. 250
Issue 6
Pg. 655-64
(Apr 10 1996)
ISSN: 0026-8925 [Print] Germany |
PMID | 8628225
(Publication Type: Journal Article)
|
Chemical References |
- DNA Primers
- Proteins
- RNA Precursors
- Chlorophyll
- Guanosine Triphosphate
|
Topics |
- Amino Acid Sequence
- Base Sequence
- Binding Sites
- Chlorella
(chemistry, genetics)
- Chlorophyll
(biosynthesis)
- Chloroplasts
(chemistry, genetics)
- Conserved Sequence
- DNA Primers
- Electrophoresis, Polyacrylamide Gel
- Genes
- Guanosine Triphosphate
(metabolism)
- Introns
(genetics)
- Molecular Sequence Data
- Nucleic Acid Conformation
- Open Reading Frames
(genetics)
- Proteins
(chemistry, genetics)
- RNA Precursors
(chemistry, metabolism)
- RNA Splicing
(genetics)
- Sequence Homology, Nucleic Acid
|