Prognostic significance of Ki-67 labeling indices obtained using MIB-1 monoclonal antibody in patients with supratentorial astrocytomas.

Abstract

BACKGROUND:

Identification of the prognosis of patients with gliomas is important for selecting and evaluating the effectiveness of treatment. The aim of this study was to evaluate the Ki-67 labeling index (LI) using the newly generated MIB-1 monoclonal antibody (MoAb) as a prognostic indicator for patients with astrocytomas.

METHODS:

Ki-67 immunohistochemistry was performed on paraffin sections to estimate the growth potential of 72 supratentorial astrocytomas using the MIB-1 MoAb after hydrated autoclaving treatment. Multivariate analysis using the Cox proportional hazard stepwise model was used to evaluate the influence of Ki-67 LI, as well as other prognostic factors, on the duration of survival of patients with supratentorial astrocytomas.

RESULTS:

The mean Ki-67 LI was 3.8% (+/- 2.7%; standard deviation [SD]) in Grade 2 gliomas (n = 19), 18.4% (+/- 9.7% SD) in Grade 3 gliomas (n = 25), and 31.6% (+/- 12.9% SD) in Grade 4 gliomas (n = 28). Multivariate survival analysis showed that histologic grade, Ki-67 LI, and Karnofsky performance status (KPS) score before and after treatment were independent, statistically significant prognostic factors for patients with all grades of astrocytomas. In high grade lesions, Ki-67 LI, tumor location (superficial vs. deep), and KPS score after treatment were significant prognostic variables, whereas histologic grade did not meet the significance level for entry into the stepwise model.

CONCLUSIONS:

These results indicate that the Ki-67 LI obtained using MIB-1 MoAb is an important and practical tool for estimating biologic behavior of gliomas, as well as for predicting survival.

• Authors: H Wakimoto, M Aoyagi, T Nakayama, G Nagashima, S Yamamoto, M Tamaki, K Hirakawa
• Journal: Cancer (Cancer) Vol. 77 Issue 2 Pg. 373-80 (Jan 15 1996) ISSN: 0008-543X [Print] United States
• PMID: 8625247 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Monoclonal
• Ki-67 Antigen
• Neoplasm Proteins
• Nuclear Proteins

Topics

• Adolescent
• Adult
• Aged
• Antibodies, Monoclonal
• Astrocytoma (diagnosis, pathology)
• Brain Neoplasms (diagnosis, pathology)
• Cell Division
• Child
• Female
• Glioblastoma (diagnosis, pathology)
• Humans
• Immunoenzyme Techniques
• Ki-67 Antigen
• Male
• Middle Aged
• Neoplasm Proteins (metabolism)
• Nuclear Proteins (metabolism)
• Prognosis