Abstract |
Suramin was found to affect the Wilms' tumor (WT) cell line, W13, by inhibiting in vitro growth (half-maximal inhibitory dose (ID50)=11 microM), insulin like growth factor II ( IGF-II) cell binding (ID50 = 10 microM) and IGF-II induced DNA synthesis (ID50 = 8 microM). In addition, suramin inhibited cross-linking of [125I] IGF-II to the type 1 IGF receptor (IGF1R) and type 2 IGF receptor (IGF2R). Disruption of IGF-II/IGF1R interaction appears to be the main mode of action of suramin since the suramin response was abolished in the presence of the IGF1R blocking antibody, alpha IR-3. When administered to athymic mice bearing W13 heterotransplants, suramin suppressed the linear tumor growth rate by 64%.
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Authors | T S Vincent, D J Hazen-Martin, A J Garvin |
Journal | Cancer letters
(Cancer Lett)
Vol. 103
Issue 1
Pg. 49-56
(May 15 1996)
ISSN: 0304-3835 [Print] Ireland |
PMID | 8616808
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Antibodies
- Antineoplastic Agents
- DNA, Neoplasm
- Receptor, IGF Type 2
- Suramin
- Insulin-Like Growth Factor II
- Receptor, IGF Type 1
- Thymidine
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Topics |
- Animals
- Antibodies
(pharmacology)
- Antineoplastic Agents
(pharmacology)
- Cell Division
(drug effects)
- Cell Line
- DNA, Neoplasm
(biosynthesis, drug effects)
- Dose-Response Relationship, Drug
- Humans
- Insulin-Like Growth Factor II
(antagonists & inhibitors, metabolism, pharmacology)
- Kidney Neoplasms
(drug therapy, pathology)
- Kinetics
- Mice
- Mice, Nude
- Mitotic Index
- Radioligand Assay
- Receptor, IGF Type 1
(antagonists & inhibitors, immunology, metabolism)
- Receptor, IGF Type 2
(antagonists & inhibitors, metabolism)
- Suramin
(pharmacology, therapeutic use)
- Thymidine
(metabolism)
- Transplantation, Heterologous
- Tumor Cells, Cultured
- Wilms Tumor
(drug therapy, pathology)
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