Nitric oxide production is increased during murine vaccinia virus infection, but may not be essential for virus clearance.

Recent reports have highlighted a potential antiviral activity for nitric oxide (NO). The purpose of this study was to investigate the production of NO in mice during vaccinia virus (VV) or herpes simplex virus type 1 infection, and to assess the role of NO in clearance of VV. Reactive nitrogen intermediates (RNI; NO and its stable oxidation products, nitrite and nitrate) were significantly elevated in the plasma of mice infected with these viruses. Furthermore, spleen cells from virus-infected mice produced elevated RNI levels following stimulation in vitro with LPS. NO production during VV infection was critically dependent on the cytokines tumor necrosis factor and interferon-gamma, and on the presence of both CD4+ and CD8+ T lymphocytes. Treatment of VV-infected mice with the nitric oxide synthase inhibitor N(G)-methyl-L-arginine did not alter the course of infection, suggesting that NO may not be essential for the clearance of this virus.
AuthorsM S Rolph, I A Ramshaw, K A Rockett, J Ruby, W B Cowden
JournalVirology (Virology) Vol. 217 Issue 2 Pg. 470-7 (Mar 15 1996) ISSN: 0042-6822 [Print] UNITED STATES
PMID8610438 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Tumor Necrosis Factor-alpha
  • Nitric Oxide
  • Interferon-gamma
  • Nitric Oxide Synthase
  • Animals
  • CD4-Positive T-Lymphocytes (immunology)
  • CD8-Positive T-Lymphocytes (immunology)
  • Cells, Cultured
  • Ectromelia virus
  • Ectromelia, Infectious (immunology)
  • Female
  • Interferon-gamma (physiology)
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred CBA
  • Nitric Oxide (biosynthesis)
  • Nitric Oxide Synthase (antagonists & inhibitors)
  • Spleen (cytology)
  • Tumor Necrosis Factor-alpha (physiology)
  • Vaccinia (blood, immunology)
  • Vaccinia virus

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