In situ hybridization studies of alpha 2-macroglobulin receptor and receptor-associated protein in human prostate carcinoma.

Abstract

We have utilized in situ hybridization and immunocytochemistry techniques to examine the expression of both alpha 2-macroglobulin (alpha 2-MR) and the 39 kDa receptor-associated protein (RAP) in 8 benign (BPH) and 34 malignant human prostate tissues, including 4 metastases. The levels of alpha 2-MR mRNA expression (but not RAP) increased significantly in high Gleason score carcinomas ( > 8) and in metastatic lesions, suggesting that alpha 2-MR expression is associated with advanced cancer. Semi-quantitative analysis with computer-assisted system analysis (CASA) confirmed this interpretation. This is the first report of alpha 2-MR expression being associated with advanced prostate cancer.

• Authors: T McGarvey, M M Hussain, M E Stearns
• Journal: The Prostate (Prostate) Vol. 28 Issue 5 Pg. 311-7 (May 1996) ISSN: 0270-4137 [Print] United States
• PMID: 8610058 (Publication Type: Journal Article)

Chemical References

• Carrier Proteins
• DNA Probes
• Glycoproteins
• LDL-Receptor Related Protein-Associated Protein
• Oligonucleotides, Antisense
• Oligoribonucleotides
• RNA, Messenger
• RNA, Neoplasm
• Receptors, Immunologic
• alpha-Macroglobulins

Topics

• Base Sequence
• Carrier Proteins (genetics, metabolism)
• DNA Probes
• Gene Expression Regulation, Neoplastic
• Glycoproteins (genetics, metabolism)
• Humans
• Immunohistochemistry
• In Situ Hybridization
• LDL-Receptor Related Protein-Associated Protein
• Male
• Molecular Sequence Data
• Oligonucleotides, Antisense (genetics)
• Oligoribonucleotides (genetics)
• Prostatic Hyperplasia (metabolism)
• Prostatic Neoplasms (chemistry, pathology)
• RNA, Messenger (analysis)
• RNA, Neoplasm (analysis)
• Receptors, Immunologic (analysis)
• Up-Regulation
• alpha-Macroglobulins (metabolism)