Expression and identification of the feline herpesvirus type 1 glycoprotein B (gp143/108).

Abstract

The gene for feline herpesvirus type 1 (FHV-1) glycoprotein B (gB) has been cloned into an expression vector, pRVSVneo, containing the long terminal repeat of Rous sarcoma virus and polyadenylation signal of SV40. This expression vector containing FHV-1 gB gene, pRVSVgBneo, was transfected into Crandell feline kidney (CRFK) cells which are susceptible to FHV-1 infection. By indirect immunofluorescence analysis, the expressed gB was recognized with a panel of monoclonal antibodies (MAbs) against FHV-1 gp143/108. Immunoprecipitation analysis using a MAb 34H12 showed that molecular weights of the gB were 143 and 108 kDa under non-denaturing conditions that 108, 70, 64, and 58 kDa under denaturing conditions. The molecular weights were similar to those of the gB expressed in FHV-1-infected CRFK cells. In addition, when plasmid DNAs were injected into mice to obtain gB-monospecific serum, the pooled serum from mice inoculated with pRVSVgBneo, but not with pRVSVgDneo or pRVSVneo, recognized the FHV-1 gB polypeptides.

• Authors: K Maeda, K Hirasawa, Y Kawaguchi, M Ono, T Mori, T Gemma, N Yokoyama, K Doi, T Mikami
• Journal: Virus research (Virus Res) Vol. 39 Issue 1 Pg. 55-61 (Nov 1995) ISSN: 0168-1702 [Print] Netherlands
• PMID: 8607284 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Monoclonal
• Antibodies, Viral
• Recombinant Proteins
• Viral Envelope Proteins
• glycoprotein B, Felid herpesvirus 1

Topics

• Animals
• Antibodies, Monoclonal (immunology)
• Antibodies, Viral (biosynthesis)
• Cats
• Cells, Cultured
• Cloning, Molecular
• Gene Expression
• Genes, Viral
• Genetic Vectors
• Herpesviridae (genetics)
• Mice
• Mice, Inbred BALB C
• Molecular Weight
• Recombinant Proteins (biosynthesis, immunology)
• Transfection (methods)
• Vaccination
• Viral Envelope Proteins (biosynthesis, genetics, immunology)