HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Molecular chaperones are present in the thylakoid lumen of pea chloroplasts.

Abstract
A soluble protein fraction was obtained from pea chloroplast thylakoids, which represents highly enriched lumenal components. Using antisera against chaperonin 60 (cpn60), chaperonin 10 (cpn10) and the heat shock cognate protein of 70 kDa (hsc70) we are able to demonstrate, that the thylakoid lumen contains a separate set of molecular chaperones, which is distinct from the stromal one. In contrast to the alpha and beta subunits of cpn60 present in the stroma the lumen contains only one cpn60 isoform of distinct isoelectric point. Furthermore the lumenal cpn10 is of 'normal' size and not like its stromal counterpart of a double-domain tandem architecture. The immunoreactive hsc70 isoforms in the lumen seem also to be different from the stromal ones. Thus, chloroplasts seem to contain the largest number of molecular chaperone isoforms present in one organelle.
AuthorsT Schlicher, J Soll
JournalFEBS letters (FEBS Lett) Vol. 379 Issue 3 Pg. 302-4 (Feb 05 1996) ISSN: 0014-5793 [Print] England
PMID8603711 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Chaperonin 10
  • Chaperonin 60
  • HSP70 Heat-Shock Proteins
  • Molecular Chaperones
  • Plant Proteins
Topics
  • Chaperonin 10 (isolation & purification)
  • Chaperonin 60 (isolation & purification)
  • Chloroplasts (chemistry)
  • HSP70 Heat-Shock Proteins (isolation & purification)
  • Molecular Chaperones (isolation & purification)
  • Pisum sativum (chemistry)
  • Plant Proteins (isolation & purification)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: