Abstract |
We determined whether aquaporin of collecting duct (AQP-CD) is involved in pathogenesis of water retention in rats with experimental models of syndrome of inappropriate secretion of antidiuretic hormone ( SIADH) and liver cirrhosis. SIADH rats were made by administering 1-desamino-8-D-arginine vasopressin ( DDAVP) subcutaneously and providing them with a liquid diet. Serum Na levels decreased to < 120 meq/l on day 2, and hyponatremia persisted throughout the rest of observation period. Six hours after the DDAVP infusion, the expression of AQP-CD mRNA significantly increased by 198%, followed by > 144% increases in its expression during the 14-day observation period. On day 7, the increased expression of AQP-CD mRNA was abolished after the administration of an antidiuretic, nonpeptide arginine vasopressin (AVP) antagonist, OPC-31260, which was closely related to a marked diuresis and a prompt normalization of serum Na levels in SIADH rats. Rats were made cirrhotic by injecting a mixture of carbon tetrachloride and olive oil subcutaneously for 3 mo. The expression of AQP-CD mRNA was increased by 164% in the decompensated cirrhotic rats. The blockade of AVP action by OPC-31260 significantly diminished its expression. These results indicate that water channel AQP-CD plays an important role in water retention in pathological states of SIADH and liver cirrhosis.
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Authors | N Fujita, S E Ishikawa, S Sasaki, G Fujisawa, K Fushimi, F Marumo, T Saito |
Journal | The American journal of physiology
(Am J Physiol)
Vol. 269
Issue 6 Pt 2
Pg. F926-31
(Dec 1995)
ISSN: 0002-9513 [Print] United States |
PMID | 8594889
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Aqp2 protein, rat
- Aquaporin 2
- Aquaporin 6
- Aquaporins
- Benzazepines
- Diuretics
- Ion Channels
- RNA, Messenger
- Water
- mozavaptan
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Topics |
- Animals
- Aquaporin 2
- Aquaporin 6
- Aquaporins
- Benzazepines
(pharmacology)
- Blotting, Northern
- Blotting, Western
- Diuretics
(pharmacology)
- Inappropriate ADH Syndrome
(metabolism)
- Ion Channels
(genetics, metabolism)
- Liver Cirrhosis, Experimental
(metabolism)
- Male
- RNA, Messenger
(metabolism)
- Rats
- Rats, Sprague-Dawley
- Water
(metabolism)
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