DNA repair in Escherichia coli: the dual function of uvr genes.

Abstract

It has been shown earlier that the starvation of E. coli for both amino-acids and thymine applied prior to UV irradiation inhibits pyrimidine dimer excision without affecting cell survival after UV irradiation. In such cells pyrimidine dimers are tolerated by a rather error-free process that depends on the activity of uvrB, recA and lexA genes. Data presented here show: (a) that the efficient toleration of unexcised dimers requires also the uvrA gene; (b) that the starvation increases the level of RecA protein about 4.7 times; (c) that the effect of starvation on subsequent pyrimidine dimer excision is reversed by a 2 h incubation in complete medium before the cells are UV irradiated. The data suggest that the uvrA, uvrB, recA, lexA dependent nonexcisional repair may be a pathway temporarily functioning in repeatedly damaged cells.

• Authors: F Masek, I Fridrichova, M Pirsel, M Sedliaková
• Journal: Neoplasma (Neoplasma) Vol. 42 Issue 6 Pg. 317-23 ( 1995) ISSN: 0028-2685 [Print] Slovakia
• PMID: 8592574 (Publication Type: Journal Article)

Chemical References

• Bacterial Proteins
• DNA, Bacterial
• DNA-Binding Proteins
• Escherichia coli Proteins
• UvrB protein, E coli
• Rec A Recombinases
• UvrA protein, E coli
• Adenosine Triphosphatases
• DNA Helicases

Topics

• Adenosine Triphosphatases (genetics)
• Bacterial Proteins (genetics)
• DNA Helicases
• DNA Repair
• DNA, Bacterial (biosynthesis)
• DNA-Binding Proteins (genetics)
• Escherichia coli (genetics)
• Escherichia coli Proteins
• Genes, Bacterial
• Rec A Recombinases (analysis)