Antiviral activity of recombinant interferon-alpha on hepatitis A virus replication in human liver cells.

Abstract

Human recombinant interferon-alpha (IFN-alpha) was assayed for its antiviral effect on hepatitis A virus (HAV) replication in the human hepatoma cell line PLC/PRF/5. IFN-alpha resulted in concentration-dependent reduction of HAV antigen expression and HAV replication. IFN-alpha had a prophylactic effect, but was still effective when it was added after the infection, even at the end of the first replication cycle. An important increase in 2',5'-oligoadenylate synthetase activity in the IFN-treated human liver cells was observed. The antiviral effect of IFN-alpha could be attributed to the induction of this enzyme. Moreover we have shown that IFN-alpha and glycyrrhizin were synergistic in their antiviral actions against HAV. IFN-alpha emerged, from the present study, as a promising candidate for chemotherapy of severe forms of hepatitis A.

• Authors: J M Crance, F Lévêque, S Chousterman, A Jouan, C Trépo, R Deloince
• Journal: Antiviral research (Antiviral Res) Vol. 28 Issue 1 Pg. 69-80 (Sep 1995) ISSN: 0166-3542 [Print] Netherlands
• PMID: 8585761 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antiviral Agents
• Hepatitis Antigens
• Interferon-alpha
• Recombinant Fusion Proteins
• Glycyrrhizic Acid
• 2',5'-Oligoadenylate Synthetase
• Glycyrrhetinic Acid

Topics

• 2',5'-Oligoadenylate Synthetase (metabolism)
• Antiviral Agents (pharmacology)
• Glycyrrhetinic Acid (analogs & derivatives, pharmacology)
• Glycyrrhizic Acid
• Hepatitis Antigens (analysis)
• Hepatovirus (drug effects, immunology, physiology)
• Humans
• Interferon-alpha (pharmacology)
• Recombinant Fusion Proteins (pharmacology)
• Time Factors
• Tumor Cells, Cultured
• Virus Replication (drug effects)