Abstract |
Retrograde perfusion of the cerebral vein (RPCV) with antioxidant LY231617 was evaluated in the focal ischemia model in rats as a new therapeutic route to deliver cytoprotective agents more selectively and efficiently into ischemic brain tissue. Thirty-six Sprague-Dawley rats were divided into Groups A through D. Focal ischemia was induced for 3 hours in the rats, then all groups were treated differently for 2 hours and then sacrificed. Rats in Group A (n = 10) served as the control group and was left untreated. Rats in Group B (n = 10) received an intravenous infusion of LY231617 (10 mg/kg/hr). Rats in Group C (n = 6) received saline (86 microliters/min) through RPCV. Rats in Group D (n = 10) received LY231617 (10 mg/kg/hr) in saline (86 microliters/min) through RPCV. The regional cerebral blood flow (rCBF) was measured using [14C] iodoantipyrine autoradiography, and phorbol 12,13-dibutyrate (PDBu) binding by in vitro [3H]PDBu autoradiography. Ischemic brain damage was assessed quantitatively after staining with cresyl violet and Luxol fast blue. Rats in Group D showed significantly higher rCBF (41-400%, p < 0.05) in the ischemic cortical and subcortical areas, and a significant reduction (66%, p < 0.01) in the total volume of ischemic damage and reduction of PDBu binding (p < 0.05) in the lateral striatum of the ischemic hemisphere, as compared to the rats in Groups A-C. RPCV with antioxidant LY231617 achieves a more beneficial effect on focal ischemic tissue than regular systemic administration.
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Authors | T Nagao, Y L Yamamoto, N Inoue, Y Ito |
Journal | Neurologia medico-chirurgica
(Neurol Med Chir (Tokyo))
Vol. 35
Issue 12
Pg. 861-8
(Dec 1995)
ISSN: 0470-8105 [Print] Japan |
PMID | 8584082
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antioxidants
- Butylated Hydroxytoluene
- LY 231617
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Topics |
- Animals
- Antioxidants
(administration & dosage, pharmacology, therapeutic use)
- Autoradiography
- Binding Sites
- Brain
(blood supply)
- Brain Ischemia
(drug therapy, physiopathology)
- Butylated Hydroxytoluene
(administration & dosage, analogs & derivatives, pharmacology, therapeutic use)
- Cerebral Veins
(drug effects)
- Infusions, Intravenous
- Rats
- Rats, Sprague-Dawley
- Regional Blood Flow
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