Automated screening system for purine and pyrimidine metabolism disorders using high-performance liquid chromatography.

Abstract

An automated screening system for purine and pyrimidine metabolism disorders using high-performance liquid chromatography (HPLC) with column switching is described. The system consists of a reversed-phase column, a cation-exchange column, a column switch, four sets of ultraviolet absorbance detectors, a microcomputer and other conventional equipment. As this system permits the simultaneous determination of urinary orotic acid, uracil, dihydrouracil, pseudouridine, xanthine, 2,8-dihydroxyadenine and succinyladenosine, it offers a useful method for the detection of orotic aciduria, dihydropyrimidine dehydrogenase deficiency, dihydropyrimidinuria, xanthinuria, adenine phosphoribosyltransferase deficiency and adenylosuccinase deficiency.

• Authors: S Sumi, K Kidouchi, S Ohba, Y Wada
• Journal: Journal of chromatography. B, Biomedical applications (J Chromatogr B Biomed Appl) Vol. 672 Issue 2 Pg. 233-9 (Oct 20 1995) ISSN: 1572-6495 [Print] Netherlands
• PMID: 8581129 (Publication Type: Journal Article)

Chemical References

• Xanthines
• dihydrouracil
• Pseudouridine
• Xanthine
• 2,8-dihydroxyadenine
• succinyladenosine
• Uracil
• Orotic Acid
• Adenine
• Adenosine

Topics

• Adenine (analogs & derivatives, urine)
• Adenosine (analogs & derivatives, urine)
• Autoanalysis
• Chromatography, High Pressure Liquid (methods, standards)
• Humans
• Orotic Acid (urine)
• Pseudouridine (urine)
• Purine-Pyrimidine Metabolism, Inborn Errors (diagnosis, urine)
• Uracil (analogs & derivatives, urine)
• Xanthine
• Xanthines (urine)