[Determination of sterigmatocystin in cancerous tissues, blood and urine in patients with liver and stomach cancer].

Abstract

Sterigmatocystin (ST) was determined with modified Southern-Western blot and indirect competitive enzyme-linked immunosorbent assay (IC-ELISA) for 28 specimens of cancerous tissues, 13 of blood and 20 of urine in 14 patients with liver and stomach cancer. Results showed DNA-ST adduct was detected in 14 specimens of cancerous tissues and/or pericancerous tissues. ST values were higher in four of 13 patients (65-113 micrograms/kg), as compared only in one of 14 healthy persons (68 micrograms/kg). And, ST values all were very low in urine, with a maximum of 13 micrograms/kg.

• Authors: H Tian, J Lou, C Du
• Journal: Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine] (Zhonghua Yu Fang Yi Xue Za Zhi) Vol. 29 Issue 5 Pg. 276-8 (Sep 1995) ISSN: 0253-9624 [Print] China
• PMID: 8556937 (Publication Type: English Abstract, Journal Article)

Chemical References

• DNA Adducts
• Sterigmatocystin

Topics

• DNA Adducts (analysis)
• Enzyme-Linked Immunosorbent Assay
• Humans
• Immunoblotting (methods)
• Liver Neoplasms (chemistry)
• Sterigmatocystin (analysis)
• Stomach Neoplasms (chemistry)