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Serodiagnosis of melioidosis in Malaysia.

Abstract
Current diagnosis of melioidosis is based on bacterial culture and/or serology which is becoming increasingly useful. An IgM-ELISA using heat-killed whole cells of Pseudomonas pseudomallei was developed and compared with an indirect haemagglutination technique (IHAT) and an indirect immunofluorescent technique(IFAT). The IgM-ELISA using a P:N ratio of > or = 2 had a sensitivity of 91% and a specificity of 96%. All 3 assays were further used in a seroepidemiological survey amongst different groups of patients and healthy individuals. It was found that the IFAT performed better than the IHAT, detecting antibodies to P. pseudomallei in 6% of diabetics, 5% of pyrexics, 8% of pregnant women and 3% of farmers. For the same groups the IgM-ELISA detected antibodies in 1% of pyrexics, 8% of pregnant women and a further 14% of farmers. The IgM-ELISA was found to be sensitive and useful for the serological diagnosis of acute melioidosis.
AuthorsJ Vadivelu, S D Puthucheary, G S Gendeh, N Parasakthi
JournalSingapore medical journal (Singapore Med J) Vol. 36 Issue 3 Pg. 299-302 (Jun 1995) ISSN: 0037-5675 [Print] SINGAPORE
PMID8553097 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
Topics
  • Adult
  • Aged
  • Case-Control Studies
  • Enzyme-Linked Immunosorbent Assay (methods)
  • Female
  • Fluorescent Antibody Technique, Indirect (methods)
  • Hemagglutination Tests (methods)
  • Humans
  • Malaysia (epidemiology)
  • Male
  • Melioidosis (blood, epidemiology, immunology, microbiology)
  • Middle Aged
  • Pregnancy
  • Prevalence
  • Sensitivity and Specificity
  • Seroepidemiologic Studies

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