Characterization of the Pseudomonas aeruginosa transposable bacteriophage D3112 A and B genes.

Abstract

The left end DNA of Mu-like transposable bacteriophage D3112 was sequenced from bp 2521 to bp 5483. Two large open reading frames were identified: ORF A (bp 2539-4611) and ORF B (bp 4626-5378). ORF A can encode a 690 amino acid, 78 kDa protein which is 44.4% similar to Mu transposase and ORF B can encode a 250 amino acid, 27 kDa protein, which is 46.4% similar to, though 62 amino acids shorter than, the Mu B protein. The cloned D3112 A gene exhibited activity on a mini-D3112-containing plasmid in Pseudomonas aeruginosa.

• Authors: P I Ulycznyj, K A Salmon, H Douillard, M S DuBow
• Journal: Biochimica et biophysica acta (Biochim Biophys Acta) Vol. 1264 Issue 3 Pg. 249-53 (Dec 27 1995) ISSN: 0006-3002 [Print] Netherlands
• PMID: 8547306 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA-Binding Proteins
• MuB protein, Enterobacteria phage Mu
• Viral Proteins
• DNA
• DNA Nucleotidyltransferases
• Transposases

Topics

• Amino Acid Sequence
• Bacteriophages (genetics)
• Base Sequence
• DNA (chemistry)
• DNA Nucleotidyltransferases (genetics)
• DNA-Binding Proteins (genetics)
• Molecular Sequence Data
• Open Reading Frames
• Plasmids
• Pseudomonas aeruginosa (genetics)
• Transposases
• Viral Proteins (genetics)