T4 DNA ligase modulates chromosome damage induced by restriction endonucleases through an error-free process.

Abstract

The possible modulation by T4 DNA ligase of the DNA double-strand breaks produced by restriction endonucleases in living mammalian cells was studied. A clear decrease in the frequency of chromosomal aberrations was observed when T4 DNA ligase was included in the electroporation treatment along with restriction enzymes inducing either cohesive- or blunt-ends in DNA. The relative proportions of exchange-type aberrations were similar for both kinds of treatments (with and without ligase), which seems to suggest an error-free ligation by T4 DNA ligase.

• Authors: T Ortiz, P Daza, J Piñero, F Cortés
• Journal: Mutagenesis (Mutagenesis) Vol. 10 Issue 5 Pg. 399-402 (Sep 1995) ISSN: 0267-8357 [Print] England
• PMID: 8544752 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Mutagens
• DNA Restriction Enzymes
• DNA Ligases

Topics

• Animals
• CHO Cells
• Chromosome Aberrations
• Chromosomes (drug effects, ultrastructure)
• Cricetinae
• Cricetulus
• DNA Damage
• DNA Ligases (pharmacology)
• DNA Repair (physiology)
• DNA Restriction Enzymes (pharmacology)
• Electroporation
• Mutagens (pharmacology)