Antisense oligodeoxynucleotides against mu- or kappa-opioid receptors block agonist-induced body temperature changes in rats.

Abstract

PL017 and dynorphin A1-17 were shown previously to cause a marked increase and a profound decrease in body temperature (Tb), respectively. In this study, we examined whether an antisense (AS) oligodeoxynucleotide (oligo) against cloned mu or kappa opioid receptors could block PL017- or dynorphin A-induced body temperature changes. Treatment with an AS oligo against mu receptors, but not sense (S) oligo, missense (MS) oligo or artificial cerebrospinal fluid (aCSF), abolished PL017-induced hyperthermia. In addition, treatment with an AS oligo against kappa receptors, but not S oligo, MS oligo or aCSF, greatly attenuated dynorphin A-induced hypothermia. This study further supports the notion that mu and kappa receptors mediate Tb regulation.

• Authors: X H Chen, E B Geller, J K de Riel, L Y Liu-Chen, M W Adler
• Journal: Brain research (Brain Res) Vol. 688 Issue 1-2 Pg. 237-41 (Aug 07 1995) ISSN: 0006-8993 [Print] Netherlands
• PMID: 8542317 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Endorphins
• Oligonucleotides, Antisense
• Receptors, Opioid, kappa
• Receptors, Opioid, mu
• Dynorphins
• morphiceptin, N-Me-Phe(3)-

Topics

• Animals
• Base Sequence
• Body Temperature Regulation (drug effects)
• Dynorphins (antagonists & inhibitors)
• Endorphins (antagonists & inhibitors)
• Injections, Intraventricular
• Molecular Structure
• Oligonucleotides, Antisense (pharmacology)
• Rats
• Receptors, Opioid, kappa (drug effects)
• Receptors, Opioid, mu (drug effects)