Effect of sulfamethazine on mixed function oxidase in chickens.

Abstract

Sulfamethazine (SMZ) ip administration for 3 d to chickens showed significant induction of cytochrome P-450 levels and in the activities of aminopyrine N-demethylase, aniline hydroxylase and glutathione s-transferase at the dosage of 150 mg SMZ/kg body weight. Cytochrome P-450 and the activity of aniline hydroxylase were significantly decreased at 300 mg SMZ/kg body weight when compared to 150 mg SMZ/kg body weight. Other doses produced no significant changes in the parameters studied. One hundred fifty mg SMZ/kg for 1 or 3 d caused significant induction of cytochrome b5, cytochrome P-450, the drug metabolizing enzymes and glutathione s-transferase; however, 5 d of SMZ produced no significant changes. The in vitro 10 mM SMZ inhibition of aminopyrine N-demethylase and aniline hydroxylase and the induction of enzymes at 150 mg SMZ/kg body weight indicates that SMZ is a substrate of the mixed function oxidase system and may be an inducer of specific forms of cytochrome P-450.

• Authors: K M Kodam, S P Govindwar
• Journal: Veterinary and human toxicology (Vet Hum Toxicol) Vol. 37 Issue 4 Pg. 340-2 (Aug 1995) ISSN: 0145-6296 [Print] United States
• PMID: 8540224 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Infective Agents
• Sulfamethazine
• Cytochrome P-450 Enzyme System
• Mixed Function Oxygenases
• Aniline Hydroxylase
• Aminopyrine N-Demethylase
• Glutathione Transferase

Topics

• Aminopyrine N-Demethylase (drug effects, metabolism)
• Aniline Hydroxylase (drug effects, metabolism)
• Animals
• Anti-Infective Agents (administration & dosage, toxicity)
• Chickens
• Cytochrome P-450 Enzyme System (biosynthesis, drug effects, metabolism)
• Enzyme Induction (drug effects)
• Glutathione Transferase (drug effects, metabolism)
• Microsomes, Liver (drug effects, enzymology)
• Mixed Function Oxygenases (drug effects, metabolism)
• Sulfamethazine (administration & dosage, toxicity)