Bleaching of melanin before image cytometry of the DNA content of pigmented skin tumors.

Abstract

OBJECTIVE:

To study the effect of bleaching of melanin with KMnO4 on the results of DNA image cytometry in pigmented skin tumors.

STUDY DESIGN:

Image cytometry of nuclear DNA content was performed on sections from 14 melanocytic skin tumors stained with Feulgen stain both with and without prior bleaching with KMnO4.

RESULTS:

The nuclear staining intensity of Feulgen stain was lower in the bleached sections, but this did not significantly affect the evaluation of ploidy. Heavy pigmentation caused some false peaks in the histograms (4 of 28 measurements made on unbleached slides).

CONCLUSION:

Bleaching of sections with KMnO4 can be useful when heavy melanin pigmentation would make DNA measurements impossible or difficult in image analysis cytometry. Bleaching is not advisable when only lightly pigmented tumors are analyzed if nuclei obscured by any pigment granules are to be avoided. In large series containing both bleached and nonbleached specimens, statistical analysis of these groups should be separated.

• Authors: L A Talve, Y U Collan, K A Alanen
• Journal: Analytical and quantitative cytology and histology (Anal Quant Cytol Histol) Vol. 17 Issue 5 Pg. 344-50 (Oct 1995) United States
• PMID: 8534337 (Publication Type: Journal Article)

Chemical References

• DNA, Neoplasm
• Melanins

Topics

• DNA, Neoplasm (analysis)
• Humans
• Image Cytometry (methods)
• Melanins
• Melanoma (genetics, pathology)
• Skin Neoplasms (genetics, pathology)
• Staining and Labeling