High prevalence of antibodies to recombinant CENP-B in primary biliary cirrhosis: nuclear immunofluorescence patterns and ELISA reactivities.

Abstract

The purpose of the present study is to evaluate the centromeric pattern on human laryngeal tumour (HEp-2) cells by indirect immunofluorescent (IIF) test and to compare their reactivities with a newly developed recombinant centromere protein B enzyme linked immunosorbent assay (CENP-B ELISA) test using sera of antinuclear antibody (ANA)-reactive primary biliary cirrhosis (PBC) patients. Antimitochondrial antibody (AMA) subtypes (PDC-E2, BCOADC-E2, OGDC, protein X, and PDC-E1 alpha) by Western blot were also investigated to see whether they have any effect on the expression of CENP-B reactivities. A centromeric pattern (anticentromere antibody [ACA]) was detected in 11 of 25 (44%) PBC patients whereas CENP-B reactivity was found in 15 (60%) of them. There were some differences in IIF patterns and CENP-B reactivities. One PBC serum with indistinguishable ANA pattern reacted with CENP-B. Eight of 15 (53%) CENP-B reactive patients had other autoimmune-like disorders. Of 181 healthy sera, none was reactive for ACA either by IIF or by ELISA test. There was a correlation between ACA IIF and CENP-B ELISA titres (r = 0.824, P < 0.001). However, no correlation was observed between either CENP-B or AMA reactivities and/or between either autoantibodies or laboratory and histologic indices of PBC. These findings suggest that recombinant CENP-B ELISA appears to be more sensitive in identifying ACA than IIF, underlying its potential value as a screening test for the diagnosis of PBC complicated with other autoimmune-like disorders. The presence of multiple autoantibodies in PBC sera may reflect heterogeneous antigens recognition, and requires further study to identify target antigens at cellular and molecular levels.

• Authors: S Parveen, S A Morshed, M Nishioka
• Journal: Journal of gastroenterology and hepatology (J Gastroenterol Hepatol) 1995 Jul-Aug Vol. 10 Issue 4 Pg. 438-45 ISSN: 0815-9319 [Print] Australia
• PMID: 8527711 (Publication Type: Journal Article)

Chemical References

• Autoantibodies
• Autoantigens
• CENPB protein, human
• Centromere Protein B
• Chromosomal Proteins, Non-Histone
• DNA-Binding Proteins

Topics

• Autoantibodies (blood)
• Autoantigens (immunology)
• Blotting, Western
• Centromere
• Centromere Protein B
• Chromosomal Proteins, Non-Histone (immunology)
• DNA-Binding Proteins
• Enzyme-Linked Immunosorbent Assay
• Female
• Fluorescent Antibody Technique, Indirect
• Humans
• Liver Cirrhosis, Biliary (immunology)
• Male
• Middle Aged
• Sensitivity and Specificity