Abstract |
To ascertain the mechanism by which nucleosomes are assembled by factors derived from Drosophila embryos, two proteins termed Drosophila chromatin assembly factors (CAFs) 1 and 4 (dCAF-1 and dCAF-4) were fractionated and purified from a Drosophila embryo extract. The assembly of chromatin by dCAF-1, dCAF-4, purified histones, ATP, and DNA is a process that generates regularly spaced nucleosomal arrays with a repeat length that resembles that of bulk native Drosophila chromatin and is not obligatorily coupled to DNA replication. The assembly of chromatin by dCAF-1 and dCAF-4 is nearly complete within 10 min. The dCAF-1 activity copurified with the Drosophila version of chromatin assembly factor-1 (CAF-1), a factor that has been found to be required for the assembly of chromatin during large tumor ( T) antigen-mediated, simian virus 40 (SV40) origin-dependent DNA replication. The dCAF-4 activity copurified with a 56-kDa core- histone- binding protein that was purified to > 90% homogeneity.
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Authors | M Bulger, T Ito, R T Kamakaka, J T Kadonaga |
Journal | Proceedings of the National Academy of Sciences of the United States of America
(Proc Natl Acad Sci U S A)
Vol. 92
Issue 25
Pg. 11726-30
(Dec 05 1995)
ISSN: 0027-8424 [Print] United States |
PMID | 8524837
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Carrier Proteins
- Chromatin
- Chromatin Assembly Factor-1
- Chromosomal Proteins, Non-Histone
- DNA-Binding Proteins
- Histones
- Nucleosomes
- Adenosine Triphosphate
- DNA
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Topics |
- Adenosine Triphosphate
(metabolism)
- Animals
- Carrier Proteins
(metabolism)
- Chromatin
(metabolism, ultrastructure)
- Chromatin Assembly Factor-1
- Chromosomal Proteins, Non-Histone
- DNA
(metabolism)
- DNA-Binding Proteins
(metabolism)
- Drosophila
- Histones
(metabolism)
- Nucleosomes
(metabolism, ultrastructure)
- Species Specificity
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