Effect of sulfo-N-succinimidyl palmitate on the rat heart: myocardial long-chain fatty acid uptake and cardiac hypertrophy.

Abstract

Abnormal long-chain fatty acid metabolism has been suggested as having a role in the genesis of certain cardiac diseases, and depressed myocardial long-chain fatty acid uptake has been clinically demonstrated in some patients with hypertrophic cardiomyopathy. However, the site where long-chain fatty acid metabolism is affected in cardiomyopathy remains unclear. Although cardiac hypertrophy is reported to be induced in rats by a fat-free diet, little is known of the consequences of depressed myocardial long-chain fatty acid uptake. Sulfo-N-succinimidyl derivatives of long-chain fatty acids have been shown to irreversibly inhibit long-chain fatty acid transport. To investigate the possible linkage of abnormal long-chain fatty acid uptake with cardiac hypertrophy, myocardial long-chain fatty acid uptake was blocked in rats using a sulfo-N-succinimidyl derivative of palmitate (SSP). SSP was intraperitoneally administered to rats for 12 weeks, and its effects on physiological parameters, and cardiac morphology were studied, SSP treatment (20 mg/kg) caused a 12% increase in heart weight (663.7 +/- 33.6 mg in controls v 741.2 +/- 26.5 mg after SSP treatment) and an 11% increase in the heart weight to body weight ratio (2.46 +/- 0.10 in controls v 2.72 +/- 0.17 after SSP) without any significant change of body weight. No significant differences were observed in blood pressure, heart rate, and serum hormones (insulin and triiodothyronine) between the control and SSP-treated groups.(ABSTRACT TRUNCATED AT 250 WORDS)

• Authors: Y Kusaka, T Tanaka, F Okamoto, F Terasaki, Y Matsunaga, H Miyazaki, K Kawamura
• Journal: Journal of molecular and cellular cardiology (J Mol Cell Cardiol) Vol. 27 Issue 8 Pg. 1605-12 (Aug 1995) ISSN: 0022-2828 [Print] England
• PMID: 8523423 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Blood Proteins
• Fatty Acids, Nonesterified
• Insulin
• Palmitates
• Succinimides
• Sulfur Radioisotopes
• Triglycerides
• sulfo-N-succinimidyl palmitate
• Triiodothyronine
• Cholesterol

Topics

• Analysis of Variance
• Animals
• Autoradiography
• Biological Transport (drug effects)
• Blood Proteins (metabolism)
• Body Weight (drug effects)
• Cardiomegaly (chemically induced, metabolism, physiopathology)
• Cholesterol (blood)
• Dose-Response Relationship, Drug
• Fatty Acids, Nonesterified (metabolism)
• Female
• Heart (drug effects, physiology, physiopathology)
• Heart Rate (drug effects)
• Insulin (blood)
• Myocardium (cytology, metabolism, pathology)
• Organ Size (drug effects)
• Palmitates (pharmacology)
• Rats
• Rats, Sprague-Dawley
• Succinimides (pharmacology)
• Sulfur Radioisotopes
• Systole (drug effects)
• Tissue Distribution
• Triglycerides (blood)
• Triiodothyronine (blood)