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Peroxidative interaction of myoglobin and myosin.

Abstract
Met-myoglobin [Fe(III)] was found to induce myosin cross-linking in the presence of H2O2 [Bhoite-Solomon, V. & Shaklai, N. (1992) Biochem. Int. 26, 181-189]. To assess the relevance of these findings to cellular pathology, peroxidation of myosin was performed with physiological divalent iron [Fe(II)] myoglobins in the oxy and deoxy forms. Both myoglobin forms were capable of mediating cross-linking of myosin. Deoxymyoglobin reactivity was similar to that of met-myoglobin, but the reactivity of oxymyoglobin was retarded compared to deoxymyoglobin. Cross-linking of myosin occurred under a low flow rate of H2O2 (3 microM/min) and in the presence of excess oxymyoglobin over H2O2, known to diminish the steady state of the myoglobin active heme [ferryl, Fe(IV)]state. The adenosinetriphosphatase activity of myosin was reduced to about half due to cross-linking. Addition of myoglobin/H2O2 to high myosin concentrations (> = 20 microM) turned the solutions into gels, a phenomenon explained by the further formation of intermolecular super cross-links of soluble myosin. Thus, at cellular ionic strength in which myosin is insoluble, cross-linking of myosin could still be triggered by myoglobin and H2O2. Based on these data, it is suggested that myoglobin-induced cross-linking of myosin and the consequent loss of adenosinetriphosphatase activity may be involved in muscle malfunction under hypoxia when cellular peroxidants increase and the deoxymyoglobin form prevails.
AuthorsT Hanan, N Shaklai
JournalEuropean journal of biochemistry (Eur J Biochem) Vol. 233 Issue 3 Pg. 930-6 (Nov 01 1995) ISSN: 0014-2956 [Print] England
PMID8521861 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Myoglobin
  • Hydrogen Peroxide
  • Adenosine Triphosphatases
  • Myosins
Topics
  • Adenosine Triphosphatases (metabolism)
  • Animals
  • Hydrogen Peroxide (metabolism)
  • Myoglobin (metabolism)
  • Myosins (metabolism)
  • Oxidation-Reduction
  • Rabbits

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