Abstract |
A transition of G to A at nucleotide position 279 in exon 1 of the vasopressin gene has been identified in patients with familial central diabetes insipidus. The mutation predicts an amino acid substitution of Thr ( ACG) for Ala (GCG) at the COOH terminus of the signal peptide in preprovasopression ( preproVP). Translation in vitro of wild-type and mutant mRNAs produced 19-kD preproVPs. When translated in the presence of canine pancreatic rough microsomes, wild-type preproVP was converted to a 21-kD protein, whereas the mutant mRNA produced proteins of 21 kD and 23 kD. NH2-terminal amino acid sequence analysis revealed that the 21-kD proteins from the wild-type and the mutants were proVPs generated by the proteolytic cleavage of the 19-residue signal peptide and the addition of carbohydrate. Accordingly, mutant preproVP was cleaved at the correct site after Thr-19, but the efficiency of cleavage by signal peptidase was < 25% that observed for the wild-type preproVP, resulting in the formation of a predominant glycosylated but uncleaved 23-kD product. These data suggest that inefficient processing of preproVP produced by the mutant allele is possibly involved in the pathogenesis of diabetes insipidus in the affected individuals.
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Authors | M Ito, Y Oiso, T Murase, K Kondo, H Saito, T Chinzei, M Racchi, M O Lively |
Journal | The Journal of clinical investigation
(J Clin Invest)
Vol. 91
Issue 6
Pg. 2565-71
(Jun 1993)
ISSN: 0021-9738 [Print] United States |
PMID | 8514868
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Amino Acids
- Membrane Proteins
- Protein Precursors
- Protein Sorting Signals
- RNA, Messenger
- Vasopressins
- Endopeptidases
- Serine Endopeptidases
- type I signal peptidase
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Topics |
- Amino Acid Sequence
- Amino Acids
(analysis)
- Asian People
- Base Sequence
- Cloning, Molecular
- Diabetes Insipidus
(genetics)
- Endopeptidases
(metabolism)
- Exons
(genetics)
- Female
- Genome
- Humans
- Japan
- Male
- Membrane Proteins
- Metabolism, Inborn Errors
(genetics)
- Molecular Sequence Data
- Mutation
(genetics)
- Pedigree
- Protein Biosynthesis
- Protein Precursors
(genetics, metabolism)
- Protein Sorting Signals
(genetics, metabolism)
- RNA, Messenger
(genetics)
- Sequence Analysis, DNA
- Serine Endopeptidases
- Transcription, Genetic
- Vasopressins
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