Abstract |
The region of DNA encoding the mannose-fucose-resistant hemagglutinin ( MFRHA) of Vibrio cholerae O1 has been localized, and the nucleotide sequence has been determined. The region contains a single open reading frame encoding 230 amino acids, corresponding to a protein of 26.9 kDa. The N terminus of this protein is atypical for a protein localized in the outer membrane. A mutant lacking MFRHA activity has been constructed by allelic exchange after inactivation via the insertion of a kanamycin resistance gene cartridge. The MFRHA-negative mutant has been assessed for virulence in the infant mouse cholera model. This mutant shows a marked defect in its ability to persist in the infant mouse gut and is incapable of competing with the wild-type organism, even when given in 25-fold excess. This defect also leads to a > 100-fold increase in the 50% lethal dose. These data suggest that the MFRHA is an important colonization factor in the infant mouse model.
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Authors | V L Franzon, A Barker, P A Manning |
Journal | Infection and immunity
(Infect Immun)
Vol. 61
Issue 7
Pg. 3032-7
(Jul 1993)
ISSN: 0019-9567 [Print] United States |
PMID | 8514410
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Hemagglutinins
- Lectins
- mannose-fucose-resistant hemagglutinin
- Fucose
- Mannose
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Topics |
- Amino Acid Sequence
- Animals
- Base Sequence
- Fucose
(pharmacology)
- Hemagglutinins
(chemistry, genetics, toxicity)
- Lectins
- Mannose
(pharmacology)
- Mice
- Molecular Sequence Data
- Mutation
- Vibrio cholerae
(genetics, pathogenicity)
- Virulence
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