Direct ABC immunohistochemistry to Encephalitozoon cuniculi.

Abstract

Among the sera from 9 rabbits spontaneously infected with Encephalitozoon cuniculi, a serum which revealed a high titer for E. cuniculi by indirect protein A-gold (IPAG) immunohistochemistry and reacted with the outer layer of the shell of E. cuniculi spores on immunoelectron microscopical examination, was biotinylated. The biotinylated rabbit anti-E. cuniculi IgG reacted immunohistochemically with E. cuniculi, but not with other protozoa tested, namely Neospora caninum, Toxoplasma gondii and sarcocystis. The direct avidin-biotin-peroxidase complex (ABC) immunohistochemistry using biotinylated rabbit anti-E. cuniculi IgG in this study is a useful tool for the diagnosis and study of encephalitozoonosis.

• Authors: J H Park, K Ochiai, C Itakura
• Journal: The Journal of veterinary medical science (J Vet Med Sci) Vol. 55 Issue 2 Pg. 325-8 (Apr 1993) ISSN: 0916-7250 [Print] Japan
• PMID: 8513018 (Publication Type: Journal Article)

Chemical References

• Antibodies, Protozoan
• Immunoglobulin G

Topics

• Animals
• Antibodies, Protozoan (blood)
• Brain (parasitology, pathology)
• Cerebral Cortex (parasitology, pathology, ultrastructure)
• Encephalitozoon cuniculi (isolation & purification)
• Encephalitozoonosis (blood, pathology, veterinary)
• Immunoglobulin G (blood)
• Immunohistochemistry
• Kidney (parasitology, pathology)
• Microscopy, Immunoelectron
• Rabbits