Protective effects of histidine during ischemia-reperfusion in isolated perfused rat hearts.

We investigated the efficacy of histidine in reducing ischemia-reperfusion (I/R)-induced myocardial injury in isolated perfused rat hearts. In I/R hearts, the contractile function and coronary flow were 59 +/- 10 and 78 +/- 6% of control. Perfusion with histidine resulted in significant increase in contractility (94 +/- 4%) and coronary flow (92 +/- 4%). The incidence of arrhythmias during reperfusion was 100% (10 out of 10) in the I/R hearts with an average duration of 12.22 +/- 1.55 (SE) min. The duration of arrhythmias was shortened to 8.24 +/- 1.46, 2.15 +/- 0.9, and 2.49 +/- 1.50 min with 10, 25, and 50 mM histidine, respectively. The duration of sinus rhythm increased from 6.26 +/- 1.56 min in I/R hearts to 10.66 +/- 1.55, 14.99 +/- 1.61, and 17.18 +/- 0.95, and 11.73 +/- 0.93 min after perfusion with 10, 25, and 50 mM histidine, and superoxide dismutase (SOD)-catalase-mannitol, respectively. Electron microscopy revealed significant ultrastructural damage of myocytes in I/R hearts, which included swelling of the mitochondria and disruption of both the sarcolemma and the myofibrils. Histidine reduced the ultrastructural damage in a dose-dependent fashion. In general, the protective effect of histidine was superior than SOD-catalase-mannitol. We conclude that histidine protects myocardium against I/R damage most likely by a singlet oxygen scavenging mechanism.
AuthorsR C Kukreja, K E Loesser, A A Kearns, S A Naseem, M L Hess
JournalThe American journal of physiology (Am J Physiol) Vol. 264 Issue 5 Pt 2 Pg. H1370-81 (May 1993) ISSN: 0002-9513 [Print] UNITED STATES
PMID8498550 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • Reactive Oxygen Species
  • Histidine
  • Animals
  • Arrhythmias, Cardiac (etiology)
  • Heart (drug effects)
  • Histidine (pharmacology)
  • In Vitro Techniques
  • Male
  • Microscopy, Electron
  • Myocardial Ischemia (complications, pathology, physiopathology)
  • Myocardial Reperfusion
  • Myocardial Reperfusion Injury (prevention & control)
  • Myocardium (ultrastructure)
  • Perfusion
  • Rats
  • Rats, Sprague-Dawley
  • Reactive Oxygen Species (metabolism)
  • Time Factors

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