Abstract |
Previous T cell receptor (TCR) sequence analysis of a panel of 23 H-2Kd restricted cytotoxic T lymphocyte (CTL) clones recognizing the decapeptide HLA-CW3 170-179 revealed a striking conservation of TCR structure, in that all clones examined used V beta 10 and J alpha pHDS58 segments. We show here that the primary response in vivo after intraperitoneal injection of DBA/2 mice with HLA-CW3 expressing transfectants of syngeneic P815 (H-2d) tumor cells is characterized by a dramatic expansion of CD8+ V beta 10+ CTL in the peritoneal cavity and draining (mesenteric) lymph node, as well as in peripheral blood. Additional analysis of TCR on HLA-CW3 immune populations by flow cytometry and polymerase chain reaction further indicates that the vast majority of responding CD8+ cells express restricted V alpha domains, a dominant J alpha segment (pHDS58), and a conserved CDR3 length for both alpha and beta chains. This novel system provides a unique opportunity to directly monitor an oligoclonal primary antigen specific immune response in vivo at the single cell level independently of functional assays.
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Authors | H R MacDonald, J L Casanova, J L Maryanski, J C Cerottini |
Journal | The Journal of experimental medicine
(J Exp Med)
Vol. 177
Issue 5
Pg. 1487-92
(May 01 1993)
ISSN: 0022-1007 [Print] United States |
PMID | 8478619
(Publication Type: Journal Article)
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Chemical References |
- CD8 Antigens
- HLA-C Antigens
- HLA-C*03 antigen
- Histocompatibility Antigens Class I
- Peptide Fragments
- Receptors, Antigen, T-Cell, alpha-beta
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Topics |
- Animals
- CD8 Antigens
- Cells, Cultured
- Clone Cells
- Female
- Flow Cytometry
- HLA-C Antigens
(immunology)
- Histocompatibility Antigens Class I
(immunology)
- Mice
- Mice, Inbred DBA
- Peptide Fragments
(immunology)
- Polymerase Chain Reaction
- Receptors, Antigen, T-Cell, alpha-beta
- T-Lymphocytes, Cytotoxic
(cytology, immunology)
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