[The chromosomal sensitivity of hematopoietic cells in Xenopus laevis larvae to the action of the chemical mutagen fotrin].

Abstract

Using the method of counting micronuclei it was shown that, in peripheral blood of larva of frog exposed to high doses of alkylating agent fotrin, the abundance of erythrocytes with signs of chromosomal aberrations significantly exceeds the level of spontaneous mutations on the 16th, 24th and 30th days of fixation but not on the 7th day following the experiment onset. In addition, with all terms of fixation under examination, an increased in comparison with norm number of binuclear erythrocytes was observed in the population of erythroid cells. Our observations combined with the data obtained by other authors confirm that amphibian hemopoietic cells can be used as a reliable test system for the detection of cyto- and genotoxic compounds as well as for the genetic monitoring of water environment.

• Authors: S T Zakhidov, Kh A Khefni, N N Svecherevskiĭ, V A Golichenkov
• Journal: Izvestiia Akademii nauk. Seriia biologicheskaia (Izv Akad Nauk Ser Biol) 1993 Jan-Feb Issue 1 Pg. 107-11 ISSN: 1026-3470 [Print] Russia (Federation)
• Vernacular Title: Chuvstvitel'nost' khromosom krovetvornykh kletok lichinok Xenopus laevis k deĭstviiu khimicheskogo mutagena fotrina.
• PMID: 8471963 (Publication Type: English Abstract, Journal Article)

Chemical References

• Aziridines
• Mutagens
• fotrin

Topics

• Animals
• Aziridines (pharmacology)
• Chromosome Aberrations
• Chromosomes (drug effects, ultrastructure)
• Dose-Response Relationship, Drug
• Hematopoietic Stem Cells (drug effects, ultrastructure)
• Larva
• Micronucleus Tests
• Mutagens (pharmacology)
• Time Factors
• Xenopus laevis (genetics)