Interactions of anti-DNA antibodies with Z-DNA.

Abstract

Systemic lupus erythematosus (SLE) sera, two classes of serum lipoproteins, and IgG antibodies from SLE and normal sera were tested for their reactivity with a Z-DNA polymer, Br-poly (dG-dC). In all cases preferential binding to Z-DNA over B-DNA was observed. This interaction, for the most part, could be inhibited by the negatively charged phospholipid, cardiolipin, which suggests that most of the anti-Z-DNA activity associated with sera arises from relatively non-specific ionic interactions between proteins and polyanionic molecules. An assay has been described that can eliminate proteins cross-reactive with negatively charged phospholipids.

• Authors: P Krishna, M J Fritzler, J H Van de Sande
• Journal: Clinical and experimental immunology (Clin Exp Immunol) Vol. 92 Issue 1 Pg. 51-7 (Apr 1993) ISSN: 0009-9104 [Print] England
• PMID: 8467564 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Antinuclear
• Cardiolipins
• DNA, Single-Stranded
• DNA-Binding Proteins
• Immunoglobulin G
• Lipoproteins
• Phosphatidic Acids
• Phosphatidylcholines
• Glycerylphosphorylcholine
• DNA

Topics

• Antibodies, Antinuclear (metabolism)
• Binding, Competitive
• Cardiolipins (physiology)
• Cross Reactions
• DNA (metabolism)
• DNA, Single-Stranded (metabolism)
• DNA-Binding Proteins (blood)
• Dose-Response Relationship, Drug
• Glycerylphosphorylcholine (pharmacology)
• Humans
• Immunoblotting
• Immunoglobulin G (metabolism)
• Lipoproteins (metabolism)
• Lupus Erythematosus, Systemic (immunology)
• Phosphatidic Acids (pharmacology)
• Phosphatidylcholines (pharmacology)