Abstract |
In the present study, it was demonstrated that there were marked strain differences in susceptibility to the induction of our new murine model of experimental autoimmune orchitis (EAO; definite orchitis with hypospermatogenesis) induced by two or three sc injections with viable syngeneic testicular germ cells (TC) without any adjuvants. Among 12 inbred strains of mice examined, the A/J (H-2a), C3H/He (H-2k), and C3H/HeN (H-2k) strains were highly susceptible, whereas the C57BL/6N (H-2b), C57BL/10Sn (H-2b), BALB/cAnN (H-2d), AKR/N (H-2k), CBA/JN (H-2k), C3H/HeJ (H-2k), and MRL/lpr (H-2k) strains were low susceptible, and the DBA/2N (H-2d) as well as C3H/BiKi (H-2k) strains were resistant. In particular, mice of the H-2k haplotype demonstrated varying degrees of susceptibility, from highly to totally resistant, to the induction of EAO. Disease susceptibility to this type of EAO does not seem to be associated with a particular H-2 haplotype. All mice of the highly susceptible strains that received two injections of TC (TC x 2) developed a significant increase in both levels of delayed footpad reaction (DFR) to TC and anti-TC antibodies measured by ELISA. In the low susceptible and the resistant strains receiving TC x 2 or TC x 3, there was no correlation between the immune responses and the susceptibility to disease in these strains, with the exception of the BALB/cAnN mice receiving TC x 3. The low susceptible and the resistant mice that received TC x 2 were classified into four groups based on the DFR and antibody response: the C57BL/6N, BALB/cAnN, CBA/JN, and C3H/HeJ strains were both positive, and the C57BL/10Sn and AKR/N strains were both negative or very low; the DBA/2N and MRL/lpr strains showed negative DFR and positive antibody response, and the C3H/BiKi strain showed quite the opposite. Almost all mice of the 12 inbred strains that received TC x 3 showed positive antibody response, although its level varied. There seems to be no linkage between the cell-mediated and humoral immune responses and the H-2 locus in our new EAO model.
|
Authors | Y Tokunaga, C Hiramine, M Itoh, A Mukasa, K Hojo |
Journal | Clinical immunology and immunopathology
(Clin Immunol Immunopathol)
Vol. 66
Issue 3
Pg. 239-47
(Mar 1993)
ISSN: 0090-1229 [Print] United States |
PMID | 8432048
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Topics |
- Animals
- Antibody Formation
- Autoimmune Diseases
(etiology)
- Disease Models, Animal
- Genetic Predisposition to Disease
- Hypersensitivity, Delayed
(immunology)
- Immunization
- Immunotherapy, Adoptive
- Male
- Mice
- Mice, Inbred Strains
(genetics)
- Orchitis
(immunology, pathology)
- Spermatozoa
(cytology, immunology)
|