Tetra-p-aminophenylporphyrin conjugated with Gd-DTPA: tumor-specific contrast agent for MR imaging.

Abstract

Tetra-p-aminophenylporphyrin (TPP) was conjugated with gadolinium diethylenetriaminepentaacetic acid (DTPA) and used as a contrast agent in magnetic resonance (MR) imaging to achieve tumor selectivity in nude mice. A substantial decrease in T1 was measured in excised tissues (kidneys, tumor, and liver) from mice that received the porphyrin derivative Gd2(DTPA)4 TPP. Toxicity and phototoxicity were less than those obtained with hematoporphyrin derivative in both L1210 lymphoblastic leukemia cells and HT 29 human colonic cancer cells, as determined with in vitro assays. MR images showed an enhancement of contrast between the tumor and adjacent tissue after injection of this agent. The results indicate that Gd2(DTPA)4TPP could be a useful prototype paramagnetic porphyrin MR imaging contrast agent with an affinity for tumors.

• Authors: F Hindré, M Le Plouzennec, J D de Certaines, M T Foultier, T Patrice, G Simonneaux
• Journal: Journal of magnetic resonance imaging : JMRI (J Magn Reson Imaging) 1993 Jan-Feb Vol. 3 Issue 1 Pg. 59-65 ISSN: 1053-1807 [Print] United States
• PMID: 8428102 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Contrast Media
• Metalloporphyrins
• Organometallic Compounds
• Porphyrins
• bis(gadolinium)-5,10,15,20-tetrakis(4-diethylenetriaminepentaacetamido)phenylporphyrin complex
• tetra-(4-aminophenyl)porphyrin
• Pentetic Acid
• Gadolinium
• Gadolinium DTPA

Topics

• Adenocarcinoma (diagnosis)
• Animals
• Colorectal Neoplasms (diagnosis)
• Contrast Media
• Gadolinium
• Gadolinium DTPA
• Humans
• Leukemia L1210 (diagnosis)
• Magnetic Resonance Imaging (methods)
• Metalloporphyrins
• Mice
• Mice, Nude
• Neoplasm Transplantation
• Organometallic Compounds
• Pentetic Acid (analogs & derivatives)
• Porphyrins