Effect of high-dose endotoxin on glucose production and utilization.

Abstract

The purpose of the present study was to determine how a high dose of endotoxin (lipopolysaccharide [LPS]), which produces hypoglycemia, alters in vivo glucose uptake by individual tissues. Catheterized conscious fasted rats were injected intravenously (i.v.) with either saline, LPS (1 mg/100 g body weight [BW], lethal dose [LD] 100), or 3-mercaptopicolinic acid (3-MP), an inhibitor of gluconeogenesis. In the latter two groups, blood glucose levels were clamped at either 6 mmol/L (euglycemia) or 3 mmol/L (hypoglycemia). In the first series of experiments, whole-body glucose flux was determined using [3-3H]glucose, and in the second study in vivo glucose uptake (Rg) by individual tissues was estimated by the tracer [U-14C]-2-deoxyglucose technique. The relative contribution of hypoglycemia per se to the LPS effect was determined by comparing the values from LPS- versus 3-MP-treated animals. There was no difference in the rate of whole-body glucose utilization (Rd) between saline-infused control rats and LPS-treated animals that were hypoglycemic. However, Rg by diaphragm, spleen, liver, and lung was increased in hypoglycemic LPS-treated rats. The increased Rg in these tissues was not observed in 3-MP-treated rats with a comparable hypoglycemia. Only the gastrocnemius muscle showed a reduction in Rg under hypoglycemic conditions, and the decrease was similar in both LPS- and 3-MP-treated animals. When sufficient glucose was infused into LPS-injected rats to maintain euglycemia, whole-body glucose Rd was increased compared with that in hypoglycemic LPS-treated rats.(ABSTRACT TRUNCATED AT 250 WORDS)

• Authors: C H Lang, Z Spolarics, A Ottlakan, J J Spitzer
• Journal: Metabolism: clinical and experimental (Metabolism) Vol. 42 Issue 10 Pg. 1351-8 (Oct 1993) ISSN: 0026-0495 [Print] United States
• PMID: 8412750 (Publication Type: Comparative Study, Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Blood Glucose
• Carbon Radioisotopes
• Endotoxins
• Insulin
• Lipopolysaccharides
• Picolinic Acids
• Tritium
• 3-mercaptopicolinic acid
• Glucagon
• Phosphoenolpyruvate Carboxykinase (GTP)
• Glucose

Topics

• Animals
• Blood Glucose (analysis)
• Carbon Radioisotopes
• Dose-Response Relationship, Drug
• Endotoxins (administration & dosage, pharmacology)
• Glucagon (analysis, blood, metabolism)
• Gluconeogenesis (drug effects)
• Glucose (metabolism)
• Hypoglycemia (blood, metabolism)
• Ileum (chemistry, metabolism)
• Injections, Intravenous
• Insulin (analysis, blood, metabolism)
• Lipopolysaccharides (pharmacology)
• Lung (chemistry, metabolism)
• Male
• Muscles (chemistry, metabolism)
• Phosphoenolpyruvate Carboxykinase (GTP) (antagonists & inhibitors)
• Picolinic Acids (pharmacology)
• Rats
• Rats, Sprague-Dawley
• Skin (chemistry, metabolism)
• Spleen (chemistry, metabolism)
• Tritium