Abstract |
The three type C retroviruses, gibbon ape leukemia virus (GALV), simian sarcoma-associated virus (SSAV), and feline leukemia virus subgroup B (FeLV-B), infect human cells by interacting with the same cell surface receptor, GLVR1. Using LacZ retroviral pseudotypes and murine cells transfected with mutant GLVR1 expression vectors, we show that the same 9-amino-acid region of human GLVR1 is critical for infection by the three viruses. Rat cells were not susceptible to infection by LacZ (FeLV-B) pseudotypes because of a block at the receptor level. We found multiple amino acid differences from human GLVR1 in the 9-amino-acid critical region of rat GLVR1. Expression of a human-rat chimeric GLVR1 in murine cells demonstrated that rat GLVR1 could function as a receptor for GALV and SSAV but not for FeLV-B. Substitution of human GLVR1 amino acids in the critical region of rat GLVR1 identified three amino acids as responsible for resistance to FeLV-B infection; two of these affect SSAV infection, but none affects GALV infection.
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Authors | C S Tailor, Y Takeuchi, B O'Hara, S V Johann, R A Weiss, M K Collins |
Journal | Journal of virology
(J Virol)
Vol. 67
Issue 11
Pg. 6737-41
(Nov 1993)
ISSN: 0022-538X [Print] United States |
PMID | 8411376
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Receptors, Virus
- Recombinant Fusion Proteins
- leukemia virus receptor, gibbon ape
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Topics |
- Amino Acid Sequence
- Animals
- DNA Mutational Analysis
- Humans
- Leukemia Virus, Feline
(growth & development)
- Leukemia Virus, Gibbon Ape
(growth & development)
- Mice
- Molecular Sequence Data
- Mutation
- Rats
- Receptors, Virus
(chemistry, genetics)
- Recombinant Fusion Proteins
(metabolism)
- Sarcoma Virus, Woolly Monkey
(growth & development)
- Sequence Alignment
- Sequence Homology, Amino Acid
- Structure-Activity Relationship
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