Abstract |
Simian-immunodeficiency-virus(SIV)-infected cynomolgus monkeys develop B-cell lymphomas in approximately one third of the cases. We have now studied the expression of cynomolgus-Epstein-Barr-virus(cyno- EBV) nuclear antigens in 13 cyno-EBV-carrying SIVsm-associated monkey lymphomas and established cell lines from 3 of these tumors. Immunoblots of cell lysates were probed with polyspecific and monospecific reagents directed against human EB-virus EBNAI-6, and against the membrane protein LMPI. An EBNA2-cross-reacting protein was demonstrated in 8 lymphoma tissues (8/13) and in the 3 cell lines derived from the tumors. All tumors expressed a polypeptide with 50 to 55 kDa molecular weight, which cross-reacted with some antibodies to EBNAI. Absorption experiments with normal monkey tissue showed that this polypeptide was specific for the cyno-EBV-carrying lymphoma cells. Equivalents of EBNA3-6 and LMPI could not be detected. Immunophenotypical characterization showed that the monkey lymphomas were similar to human HIV-associated B-cell lymphomas. Malignant B-cell lymphomas in experimentally SIVsm-infected cynomolgus monkeys can be a model for EBV-associated lymphomagenesis in immunodeficiency states.
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Authors | S L Li, H Feichtinger, E Kaaya, P Migliorini, P Putkonen, G Biberfeld, J M Middeldorp, P Biberfeld, I Ernberg |
Journal | International journal of cancer
(Int J Cancer)
Vol. 55
Issue 4
Pg. 609-15
(Oct 21 1993)
ISSN: 0020-7136 [Print] United States |
PMID | 8406989
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antigens, CD
- Antigens, Nuclear
- Antigens, Viral
- DNA-Binding Proteins
- Epstein-Barr Virus Nuclear Antigens
- Nuclear Proteins
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Topics |
- Animals
- Antigens, CD
(analysis)
- Antigens, Nuclear
- Antigens, Viral
(metabolism)
- Blotting, Western
- Cross Reactions
- DNA-Binding Proteins
(metabolism)
- Epstein-Barr Virus Nuclear Antigens
- Herpesvirus 4, Human
(immunology)
- Immunohistochemistry
- Lymphoma, B-Cell
(immunology)
- Macaca fascicularis
- Nuclear Proteins
(analysis)
- Simian Immunodeficiency Virus
(immunology)
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