Lectin binding and expression of blood group-related antigens in carcinoma-in-situ and invasive carcinoma of urinary bladder.

Abstract

To determine whether histochemical reactivities of carcinoma-in-situ of the urinary bladder differ from those of invasive transitional cell carcinoma, we tested a profile of eight different lectins and three antibodies directed against blood group-related antigens for 15 cases of carcinoma-in-situ and 26 cases of non-papillary (6 superficially and 20 deeply) invasive transitional cell carcinoma that had been diagnosed according to the histopathological criteria of the International Union against Cancer. For biotin-labelled lectins and monoclonal antibodies to mouse blood group-related antigens, the avidin-biotin peroxidase complex method was applied. Positive histochemical reactions of peanut agglutinin without neuraminidase treatment--PNA N(-)--in the 20 deeply invasive tumour cases were significantly higher than those in the 15 carcinoma-in-situ cases (P < 0.05). In contrast, the reactions of blood group-related antigens in the 20 deeply invasive tumour cases were significantly lower than those in the 15 carcinoma-in-situ cases or the 11 normal controls (P < 0.05). The results confirm previously reported studies of the staining of PNA N(-) and blood group-related antigens on carcinoma-in-situ and invasive tumours of urothelial organs. The application of lectins and blood group-related antigens to the histopathology of urinary bladder cancer may be helpful in the differential diagnosis of carcinoma-in-situ from invasive cancer, but neither PNA N(-) nor blood group-related antigens can be solely reliable in this.

• Authors: K Nakanishi, T Kawai, M Suzuki
• Journal: Histopathology (Histopathology) Vol. 23 Issue 2 Pg. 153-8 (Aug 1993) ISSN: 0309-0167 [Print] England
• PMID: 8406387 (Publication Type: Journal Article)

Chemical References

• Blood Group Antigens
• Isoantigens
• Lectins

Topics

• Blood Group Antigens (immunology)
• Carbohydrate Sequence
• Carcinoma in Situ (pathology)
• Carcinoma, Transitional Cell (pathology)
• Humans
• Immunoenzyme Techniques
• Isoantigens (analysis)
• Lectins (metabolism)
• Molecular Sequence Data
• Urinary Bladder Neoplasms (pathology)