Peritoneal sclerosis: role of plasticizers in stimulating interleukin-1 production.
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| Abstract |
The role of plasticizers (PLS) in inducing water flow inhibition and peritoneal sclerosis has been demonstrated in both in vivo and in vitro studies. Interleukin-1 (IL-1) has been shown to be a regulator of fibroblast proliferation as well as collagenase production. The aim of this study was to evaluate the role of PLS in stimulating mononuclear cell IL-1 secretion. Two cultures containing 10(3) cells/mL were obtained from 14 healthy subjects. One was used as the control, and the other was mixed with diethylhexylphthalate (DEHP) to reach a final concentration of 2.8 x 10(-3) M. After 4 hours the samples were centrifuged, and the supernatants were tested by radioimmunoassay for IL-1 alpha and IL-1 beta. The results showed a significant increase in both IL-1 alpha and IL-1 beta production in DEHP-stimulated cells in comparison to the controls: 42.6 +/- 15.4 versus 29.3 +/- 10 ng/L (p < 0.015) for IL-1 alpha, and 153.6 +/- 55 versus 113.6 +/- 32 ng/L (p < 0.03) for IL-1 beta In conclusion, PLS added to mononuclear cells were able to induce IL-1 secretion. This mechanism could be responsible, at least in part, for the development of peritoneal sclerosis. Thus the employment of plasticizer-free bags should be elective in peritoneal dialysis.
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| Authors | A Fracasso, L Calo, S Landini, P Morachiello, F Righetto, F Scanferla, P Toffoletto, R Genchi, D Roncali, S Cantaro |
| Journal | Peritoneal dialysis international : journal of the International Society for Peritoneal Dialysis (Perit Dial Int) Vol. 13 Suppl 2 Pg. S517-9 ( 1993) ISSN: 0896-8608 [Print] United States |
| PMID | 8399653 (Publication Type: Journal Article) |
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