Abstract |
Posttransplant lymphoproliferative disorders in organ allograft recipients are most commonly of B cell origin, whereas T cell lymphomas are rarely described. We report a case of T cell immunoblastic large cell lymphoma associated with Epstein-Barr virus (EBV) that occurred in a recipient of a cadaveric renal transplant 7 years posttransplantation. On paraffin immunophenotyping, none of the neoplastic cells stained with the T cell-associated markers used, but did show strong CD30 expression. Flow cytometric studies revealed a predominance of T cells without definite evidence of T cell neoplasia. Frozen section immunophenotyping studies revealed a T cell phenotype with aberrant expression, and genotypic studies demonstrated T cell receptor beta gene rearrangement with germline configuration of immunoglobulin heavy chain and kappa light chain genes, confirming a T lineage. EBV-encoded RNA transcripts were demonstrated within the neoplastic cells by in situ hybridization. Southern blot analysis using probes derived from the terminal repeat region of the virus detected a single restriction band indicating a clonal population. We believe this is the first case of a posttransplant T cell lymphoma in which the EBV genome has been demonstrated. This case also illustrates the pitfalls of paraffin immunophenotyping in the diagnosis of T cell lymphoma.
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Authors | S Kumar, D Kumar, D W Kingma, E S Jaffe |
Journal | The American journal of surgical pathology
(Am J Surg Pathol)
Vol. 17
Issue 10
Pg. 1046-53
(Oct 1993)
ISSN: 0147-5185 [Print] United States |
PMID | 8396856
(Publication Type: Case Reports, Journal Article)
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Topics |
- Adult
- Blotting, Southern
- Herpesvirus 4, Human
(isolation & purification)
- Humans
- Immunophenotyping
- In Situ Hybridization
- Kidney Transplantation
(adverse effects)
- Male
- Precursor Cell Lymphoblastic Leukemia-Lymphoma
(etiology, immunology, pathology)
- Transplantation Immunology
- Tumor Virus Infections
(etiology, immunology, pathology)
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