Protective activity of the lipid A analogue GLA-60 against murine cytomegalovirus infection in immunodeficient mice.

Abstract

The immunomodulating and murine cytomegalovirus (MCMV)-inhibiting effects of the synthetic lipid A subunit analogue GLA-60 were investigated in different strains of immunodeficient mice. Peritoneal natural killer (NK) cells obtained from nude (nu/nu) C57BL/6 mice or normal NMRI mice, which had been treated intraperitoneally with 10 micrograms of GLA-60 1 day earlier, exhibited a greater cytolytic activity than those from untreated mice. GLA-60 also stimulated NK cell activity in SCID (severe combined immune deficiency) mice (which are T and B cell-defective), but not in NK cell-defective beige (C57BL/6 bg/bg) mice. GLA-60 also enhanced the phagocytic activity of peritoneal macrophages in beige, nude and NMRI mice, but not in SCID mice. GLA-60, when administered as a single 150 micrograms dose 1 day before infection, completely protected beige mice against MCMV-associated mortality. It also caused a significant increase in the life-span of MCMV-infected nude and SCID mice.

• Authors: S Ikeda, J Neyts, M Matsuura, M Kiso, A Hasegawa, C Nishimura, E De Clercq
• Journal: The Journal of general virology (J Gen Virol) Vol. 74 ( Pt 7) Pg. 1399-403 (Jul 1993) ISSN: 0022-1317 [Print] England
• PMID: 8393081 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antiviral Agents
• Lipid A
• GLA 60

Topics

• Animals
• Antiviral Agents (pharmacology)
• Cytomegalovirus (immunology)
• Cytomegalovirus Infections (immunology, prevention & control)
• Killer Cells, Natural (immunology)
• Lipid A (analogs & derivatives, pharmacology)
• Macrophages (drug effects, immunology)
• Mice
• Mice, Inbred C57BL (immunology)
• Mice, Inbred Strains (immunology)
• Mice, Nude (immunology)
• Mice, SCID (immunology)
• Phagocytosis (drug effects)
• Species Specificity